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Effects of chlorogenic acid on intracellular calcium regulation in lysophosphatidylcholine-treated endothelial cells

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Author(s)
Hye-Jin JungSeung-Soon ImDae-Kyu SongJae-Hoon Bae
Keimyung Author(s)
Song, Dae KyuIm, Seung SoonBae, Jae Hoon
Department
Dept. of Physiology (생리학)
Journal Title
BMB Reports
Issued Date
2017
Volume
50
Issue
6
Keyword
CalciumChlorogenic acidLysophosphatidylcholineStore-operated channelTransient receptor potential canonical channel 1
Abstract
Lysophosphatidylcholine (LPC) is a major phospholipid component of oxidized low-density lipoprotein (ox-LDL) and is implicated in its atherogenic activity. This study investigated the effects of LPC on cell viability, intracellular calcium homeostasis, and the protective mechanisms of chlorogenic acid (CGA) in human umbilical vein endothelial cells (HUVECs). LPC increased intracellular calcium ([Ca2+]i) by releasing Ca2+ from intracellular stores and via Ca2+ influx through store-operated channels (SOCs). LPC also increased the generation of reactive oxygen species (ROS) and decreased cell viability. The mRNA expression of Transient receptor potential canonical (TRPC) channel 1 was increased significantly by LPC treatment and suppressed by CGA. CGA inhibited LPC-induced Ca2+ influx and ROS generation, and restored cell viability. These results suggested that CGA inhibits SOC-mediated Ca2+ influx and ROS generation by attenuating TRPC1 expression in LPC-treated HUVECs. Therefore, CGA might protect endothelial cells against LPC injury, thereby inhibiting atherosclerosis.
Keimyung Author(s)(Kor)
송대규
임승순
배재훈
Publisher
School of Medicine
Citation
Hye-Jin Jung et al. (2017). Effects of chlorogenic acid on intracellular calcium regulation in lysophosphatidylcholine-treated endothelial cells. BMB Reports, 50(6), 323–328. doi: 10.5483/BMBRep.2017.50.6.182
Type
Article
ISSN
1976-670X
DOI
10.5483/BMBRep.2017.50.6.182
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/32366
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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