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The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis

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Author(s)
Jung-Won ShinHye-Ryung ChoiKyung-Mi NamHyun-Sun LeeSung-Ae KimHyun-Jae JoeToyama KazumiKyoung-Chan Park
Keimyung Author(s)
Kim, Sung Ae
Department
Dept. of Dermatology (피부과학)
Journal Title
International Journal of Molecular Sciences
Issued Date
2017
Volume
18
Issue
7
Keyword
epidermal stem cellHDAC1interfollicular epidermisstem cell nichep63suberoylanilohydroxamic acid (SAHA)
Abstract
Stem cell markers of interfollicular epidermis (IEF) have not been established thus far. The aim of this study is to suggest a new way to disclose IFE-stem cells by combining the expression of histone deacetylases (HDAC) 1 and p63. Immunohistochemical staining of HDAC1 and p63 was performed in six normal human samples. Moreover, a skin equivalent (SE) model was treated with suberoylanilohydroxamic acid (SAHA, an HDAC inhibitor) to elucidate the role of HDAC1. Finally, rapidly adhering (RA) keratinocytes to a type IV collagen, which have been identified to represent epidermal stem cells, were subjected to Western blot analysis with antibodies against HDAC1. In normal samples, there was a minor subpopulation comprised of p63-positive and HDAC1-negative cells in the basal layers. The proportion of this subpopulation was decreased with age. In the SE model, SAHA treatment increased the epidermal thickness and number of p63-positive cells in a dose dependent manner. After SAHA treatment, the expression of differentiation markers was decreased, while that of basement membrane markers was increased. In a Western blot analysis, HDAC1 was not expressed in RA cells. In conclusion, the combination of p63-positive and HDAC1-negative expressions can be a potential new way for distinguishing epidermal stem cells.
Keimyung Author(s)(Kor)
김성애
Publisher
School of Medicine
Citation
Jung-Won Shin et al. (2017). The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis. International Journal of Molecular Sciences, 18(7), 1360–1360. doi: 10.3390/ijms18071360
Type
Article
ISSN
1422-0067
DOI
10.3390/ijms18071360
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/32424
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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