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The effect of salinomycin on ovarian cancer stem-like cells

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Author(s)
Hyewon Chung, Yu-Hwan Kim, Myoung Kwon, So-Jin Shin, Sang-Hoon Kwon, Soon-Do ChaChi-Heum Cho
Keimyung Author(s)
Cha, Soon DoKwon, Sang HoonShin, So JinCho, Chi Heum
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Journal Title
Obstetrics & Gynecology Science
Issued Date
2016
Volume
59
Issue
4
Keyword
Ovarian neoplasmsSalinomycinStem-like cell
Abstract
Objective;

The identification of cancer stem-like cells is a recent development in ovarian cancer. Compared to other cancer cells, cancer stem-like cells present more chemo-resistance and more aggressive characteristics. They play an important role in the recurrence and drug resistance of cancer. Therefore, the target therapy of cancer stem-like cell may become a promising and effective approach for ovarian cancer treatment. It may also help to provide novel diagnostic and therapeutic strategies.


Methods;

The OVCAR3 cell line was cultured under serum-free conditions to produce floating spheres. The CD44+CD117+ cell line was isolated from the human ovarian cancer cell line OVCAR3 by using immune magnetic-activated cell sorting system. The expression of stemness genes such as OCT3/4, NANOG and SOX2 mRNA were determined by reverse transcription polymerase chain reaction. OVCAR3 parental and OVCAR3 CD44+CD117+ cells were grown in different doses of paclitaxel and salinomycin to evaluate the effect of salinomycin. And growth inhibition of OVCAR3 CD44+CD117+ cells by paclitaxel combined with salinomycin was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.


Results;

Tumor spheroids generated from the OVCAR3 cell line are shown to have highly enriched CD44 and CD117 expression. Treatment with a combination of paclitaxel and salinomycin demonstrated growth inhibition of OVCAR3 CD44+CD117+ cells.


Conclusion;

The present study is a detailed investigation on the expression of CD44 and CD117 in cancer stem cells and evaluates their specific tumorigenic characteristics in ovarian cancer. This study also demonstrates significant growth inhibition of cancer stem-like cells by paclitaxel combined with salinomycin. Identification of these cancer stem-like cell markers and growth inhibition effect of salinomycin may be the next step to the development of novel target therapy in ovarian cancer.
Keimyung Author(s)(Kor)
차순도
권상훈
신소진
조치흠
Publisher
School of Medicine
Citation
Hyewon Chung, Yu-Hwan Kim, Myoung Kwon, So-Jin Shin, Sang-Hoon Kwon, Soon-Do Cha and Chi-Heum Cho. (2016). The effect of salinomycin on ovarian cancer stem-like cells. Obstetrics & Gynecology Science, 59(4), 261–268. doi: 10.5468/ogs.2016.59.4.261
Type
Article
ISSN
2287-8572
DOI
10.5468/ogs.2016.59.4.261
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/32533
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
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