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Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus

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Affiliated Author(s)
박재형황일선조광범박경식
Alternative Author(s)
Hwang, Il SeonPark, Jae HyungCho, Kwang BumPark, Kyung Sik
Journal Title
World Journal of Gastroenterology
ISSN
1007-9327
Issued Date
2016
Keyword
Diabetes mellitusInterstitial cells of CajalFibroblast-like cellGastroparesisEnteric nerve system
Abstract
AIM;

To investigate histologic abnormalities in the gastric smooth muscle of patients with diabetes mellitus (DM).


METHODS;

Full-thickness gastric specimens were obtained from patients undergoing surgery for gastric cancer. H&E stain and Masson’s Trichrome stain were performed to assess the degree of fibrosis. Immunohistochemical staining using various antibodies was also performed [antibodies against protein gene product 9.5 (PGP9.5), neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP), neurokinin-1 (NK1) receptor, c-Kit, and platelet-derived growth factor receptor-alpha, (PDGFRα)]. Immunofluorescent staining and evaluation with confocal microscopy were also conducted.


RESULTS;

Twenty-six controls and 35 diabetic patients (21 short-duration patients and 14 long-duration patients) were included. There were no significant differences in basic demographics between the two groups except in mean body mass index (BMI) (higher in the DM group). Proportions of moderate-to-severe intercellular fibrosis in the muscle layer were significantly higher in the DM group than in the control group (P < 0.01). On immunohistochemical staining, c-Kit- and PDGFRα-positive immunoreactivity were significantly decreased in the DM group compared with the control group (P < 0.05). There were no statistically significant differences in PGP9.5, nNOS, VIP, and neurokinin 1 expression. On immunofluorescent staining, cellularity of interstitial cells of Cajal (ICC) was observed to decrease with increasing duration of DM.


CONCLUSION;

Our study suggests that increased intercellular fibrosis, loss of ICC, and loss of fibroblast-like cells are found in the smooth muscle of DM patients. These abnormalities may contribute to changes in gastric motor activity in patients with DM.
Department
Dept. of Pathology (병리학)
Publisher
School of Medicine
Citation
Kyung Sik Park et al. (2016). Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus. World Journal of Gastroenterology, 22(46), 10131–10139. doi: 10.3748/wjg.v22.i46.10131
Type
Article
ISSN
1007-9327
DOI
10.3748/wjg.v22.i46.10131
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/32585
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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