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Variable alterations of mitochondrial microsatellite instability and DNA copy number in pulmonary hamartomas.

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Author(s)
Lee, Deok HeonLee, Jae-HoKeum, Dong YoonKim, Dae-Kwang
Keimyung Author(s)
Keum, Dong YoonKim, Dae KwangLee, Jae Ho
Department
Dept. of Anatomy (해부학)
Dept. of Medical Genetics (의학유전학)
Journal Title
Cancer Biomarkers
Issued Date
2016
Volume
17
Issue
4
Keyword
Hamartomamicrosatellite instabilityDNA copy number
Abstract
BACKGROUND: The genetic alteration of mitochondrial DNA has been regarded as an important step in the development of several human tumors. OBJECTIVE: The purpose of this study was to identify frequency of mitochondrial microsatellite instability (mtMSI) and alterations in mitochondrial DNA copy number (mtCN) in pulmonary hamartoma. METHODS: DNA was isolated from tumor tissue and matched non-tumor tissue in 30 patients with pulmonary hamartoma. BAT 25 and 26 were used as nucleus MSI (nMSI) markers, and (C)n and (CA)n in D-loop were used as mtMSI markers. MtCNs were quantified using a competitive quantitative real-time polymerase chain reaction. RESULTS: nMSI was detected in 5 patients (23.8%) and mtMSI was detected in 2 patients (9.5%) of total 21 hamartoma. There were 14 patients (46.7%), 2 patients (6.7%), and a further 14 patients (46.7%) in the decreased, no change, and increased mtCN groups, respectively. The mean relative mtCN were 0.4 ± 0.3 in the decreased and 3.9 ± 5.1 in the increased mtCN groups, respectively. CONCLUSIONS: nMSI was more frequently appeared than mtMSI in hamartomas, and we also found measurements of mtCNs in patients with pulmonary hamartoma to be extremely variable without any characteristic pattern.
Keimyung Author(s)(Kor)
금동윤
김대광
이재호
Publisher
School of Medicine
Citation
Lee, Deok Heon et al. (2016). Variable alterations of mitochondrial microsatellite instability and DNA copy number in pulmonary hamartomas. Cancer Biomarkers, 17(4), 473–478. doi: 10.3233/CBM-160664
Type
Article
ISSN
1574-0153
Source
https://content.iospress.com/articles/cancer-biomarkers/cbm664
DOI
10.3233/CBM-160664
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/32690
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
1. School of Medicine (의과대학) > Dept. of Medical Genetics (의학유전학)
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