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Establishing Quality Control Ranges for Antimicrobial Susceptibility Testing of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus: A Cornerstone to Develop Reference Strains for Korean Clinical Microbiology Laboratories

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Author(s)
Sung Kuk HongSeung Jun ChoiSaeam ShinWonmok LeeNaina PintoNari ShinKwangjun LeeSeong Geun HongYoung Ah KimHyukmin LeeHeejung KimWonkeun SongSun Hwa LeeDongeun YongKyungwon LeeYunsop Chong
Keimyung Author(s)
Lee, Won Mok
Department
Dept. of Laboratory Medicine (진단검사의학)
Journal Title
Annals of Laboratory Medicine.
Issued Date
2015
Volume
35
Issue
6
Keyword
Agar dilution testAntimicrobial susceptibility testClinical microbiology laboratoryDisk diffusion testQuality controlReference strain
Abstract
Quality control (QC) processes are being performed in the majority of clinical microbiology laboratories to ensure the performance of microbial identification and antimicrobial susceptibility testing by using ATCC strains. To obtain these ATCC strains, some inconveniences are encountered concerning the purchase cost of the strains and the shipping time required. This study was focused on constructing a database of reference strains for QC processes using domestic bacterial strains, concentrating primarily on antimicrobial susceptibility testing. Three strains (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) that showed legible results in preliminary testing were selected. The minimal inhibitory concentrations (MICs) and zone diameters (ZDs) of eight antimicrobials for each strain were determined according to the CLSI M23. All resulting MIC and ZD ranges included at least 95% of the data. The ZD QC ranges obtained by using the CLSI method were less than 12 mm, and the MIC QC ranges extended no more than five dilutions. This study is a preliminary attempt to construct a bank of Korean QC strains. With further studies, a positive outcome toward cost and time reduction can be anticipated.
Keimyung Author(s)(Kor)
이원목
Publisher
School of Medicine
Citation
Sung Kuk Hong et al. (2015). Establishing Quality Control Ranges for Antimicrobial Susceptibility Testing of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus: A Cornerstone to Develop Reference Strains for Korean Clinical Microbiology Laboratories. Annals of Laboratory Medicine., 35(6), 635–638. doi: 10.3343/alm.2015.35.6.635
Type
Article
ISSN
2234-3806
DOI
10.3343/alm.2015.35.6.635
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/32964
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
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