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Inhibitory effect of 1,2,4,5-tetramethoxybenzene on mast cell-mediated allergic inflammation through suppression of IκB kinase complex.

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Author(s)
In-Gyu JeHyun Gyu ChoiHui-Hun KimSoyoung LeeJin Kyeong ChoiSung-Wan KimDuk-Sil KimTaeg Kyu KwonTae-Yong ShinPil-Hoon ParkDongwoo KhangSang-Hyun Kim
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Toxicology and Applied Pharmacology.
Issued Date
2015
Volume
287
Issue
2
Keyword
Allergic inflammationMast cellsHistaminePro-inflammatory cytokineActive systemic anaphylaxis
Abstract
As the importance of allergic disorders such as atopic dermatitis and allergic asthma, research on potential drug
candidates becomesmore necessary.Mast cells play an important role as initiators of allergic responses through
the release of histamine; therefore, they should be the target of pharmaceutical development for the management
of allergic inflammation. In our previous study, anti-allergic effect of extracts of Amomum xanthioides
was demonstrated. To further investigate improved candidates, 1,2,4,5-tetramethoxybenzene (TMB) was isolated
from methanol extracts of A. xanthioides. TMB dose-dependently attenuated the degranulation of mast cells
without cytotoxicity by inhibiting calcium influx. TMB decreased the expression of pro-inflammatory cytokines
such as tumor necrosis factor-α and interleukin (IL)-4 at both the transcriptional and translational levels.
Increased expression of these cytokines was caused by translocation of nuclear factor-κB into the nucleus, and
it was hindered by suppressing activation of IκB kinase complex. To confirmthe effect of TMB in vivo, the ovalbumin
(OVA)-induced active systemic anaphylaxis (ASA) and IgE-mediated passive cutaneous anaphylaxis (PCA)
models were used. In the ASAmodel, hypothermia was decreased by oral administration of TMB, which attenuated
serum histamine, OVA-specific IgE, and IL-4 levels. Increased pigmentation of Evans blue was reduced by
TMB in a dose-dependent manner in the PCA model. Our results suggest that TMB is a possible therapeutic
candidate for allergic inflammatory diseases that acts through the inhibition of mast cell degranulation and
expression of pro-inflammatory cytokines.
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
In-Gyu Je et al. (2015). Inhibitory effect of 1,2,4,5-tetramethoxybenzene on mast cell-mediated allergic inflammation through suppression of IκB kinase complex. Toxicology and Applied Pharmacology., 287(2), 119–127. doi: 10.1016/j.taap.2015.05.006
Type
Article
ISSN
0041-008X
Source
https://www.sciencedirect.com/science/article/pii/S0041008X15001702?via%3Dihub
DOI
10.1016/j.taap.2015.05.006
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/32981
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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