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Gastroprotective Effects of PMK-S005 against Ethanol-Induced Acute Gastric Damage in Rats.

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Author(s)
Yoon Jeong ChoiNayoung KimJu Yup LeeRyoung Hee NamJi Hyung SeoSeonmin LeeHee Jin KimYoon Jin ChoiHye Seung LeeDong Ho Lee
Keimyung Author(s)
Lee, Ju Yup
Department
Dept. of Internal Medicine (내과학)
Journal Title
Gut and Liver
Issued Date
2016
Volume
10
Issue
3
Keyword
S-allyl-L-cysteineGastroprotectionAnti-inflammationAntioxidantsEthanol
Abstract
Background/Aims: This study aimed to examine the gastroprotective
effects of PMK-S005, which is a synthetic S-allyl-Lcysteine
(SAC; a sulfur-containing amino acid), against acute
ethanol-induced gastric damage in rats. Methods: Sprague-
Dawley rats were divided into six groups, including a nonethanol
group, groups treated with absolute ethanol 1 hour after
pretreatment with various doses of PMK-S005 (1, 5, and 10
mg/kg) or rebamipide (50 mg/kg), and an absolute ethanolonly
group. Ethanol-induced gross ulcer and mucus levels
were measured. Myeloperoxidase, tumor necrosis factor a,
interleukin 1β, PGE2, LTB4, cPLA2, COX-1, and COX-2 levels
were estimated by enzyme-linked immunosorbent assay or
Western blot analysis. Furthermore, the protein expression
levels of antioxidant enzymes, including heme oxygenase-1
(HO-1), NAD(P)H:quinine oxidoreductase 1 (NQO-1), GCLC,
and GCLM, were assessed. Results: PMK-S005 significantly
attenuated the ethanol-induced gastric damage; it reduced
mucosal inflammatory cytokine production and increased
mucus levels. The expression levels of cPLA2, COX-1, and
COX-2 were decreased by PMK-S005. PMK-S005 did not
affect PGE2 synthesis, but LTB4 production was significantly
suppressed. In addition, long-term administration of PMKS005
significantly increased the expression of HO-1, NQO-1,
GCLC, and GCLM. Conclusions: These results strongly suggest
that PMK-S005 prevents gastric mucosal damage and
that these gastroprotective activities are due to anti-inflammatory
effects and enhancement of the gastric defense system,
including antioxidant enzymes.
Keimyung Author(s)(Kor)
이주엽
Publisher
School of Medicine
Citation
Yoon Jeong Choi et al. (2016). Gastroprotective Effects of PMK-S005 against Ethanol-Induced Acute Gastric Damage in Rats. Gut and Liver, 10(3), 348–355. doi: 10.5009/gnl14509
Type
Article
ISSN
1976-2283
DOI
10.5009/gnl14509
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33207
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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