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Glucosamine suppresses platelet-activating factor-induced activation of microglia through inhibition of store-operated calcium influx

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Author(s)
Jae-Hyung ParkJeong-Nam KimByeong-Churl JangSeung-Soon ImDae-Kyu SongJae-Hoon Bae
Keimyung Author(s)
Park, Jae HyungIm, Seung SoonSong, Dae KyuBae, Jae HoonJang, Byeong Churl
Department
Dept. of Physiology (생리학)
Dept. of Molecular Medicine (분자의학)
Journal Title
Environmental Toxicology and Pharmacology.
Issued Date
2016
Volume
42
Keyword
GlucosaminePlatelet-activating factorMicrogliaIntracellular calcium concentrationStore-operated calcium channel
Abstract
Microglia activation and subsequent release of inflammatory mediators are implicated in the pathophysi-ology of neurodegenerative diseases. Platelet-activating factor (PAF), a potent lipid mediator synthesizedby microglia, is known to stimulate microglia functional responses. In this study, we determined thatendogenous PAF exert autocrine effects on microglia activation, as well as the underlying mechanisminvolved. We also investigated the effect of d-glucosamine (GlcN) on PAF-induced cellular activationin human HMO6 microglial cells. PAF induced sustained intracellular Ca2+([Ca2+]i) increase throughstore-operated Ca2+channels (SOC) and reactive oxygen species (ROS) generation. PAF also induced pro-inflammatory markers through NF B/COX-2 signaling. GlcN significantly inhibited PAF-induced Ca2+influx and ROS generation without significant cytotoxicity. GlcN downregulated excessive expression ofpro-inflammatory markers and promoted filopodia formation through NF B/COX-2 inhibition in PAF-stimulated HMO6 cells. Taken together, these data suggest that GlcN may offer substantial therapeuticpotential for treating inflammatory and neurodegenerative diseases accompanied by microglial activa-tion.
Keimyung Author(s)(Kor)
박재형
임승순
송대규
배재훈
장병철
Publisher
School of Medicine
Citation
Jae-Hyung Park et al. (2016). Glucosamine suppresses platelet-activating factor-induced activation of microglia through inhibition of store-operated calcium influx. Environmental Toxicology and Pharmacology., 42, 1–8. doi: 10.1016/j.etap.2015.12.014
Type
Article
ISSN
1382-6689
Source
https://linkinghub.elsevier.com/retrieve/pii/S1382-6689(15)30155-1
DOI
10.1016/j.etap.2015.12.014
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33210
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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