계명대학교 의학도서관 Repository

Grape seed proanthocyanidin extract ameliorates murine autoimmune arthritis through regulation of TLR4/MyD88/NF-κB signaling pathway.

Metadata Downloads
Author(s)
Sang-Hyon KimJihye BangChang-Nam SonWon-Ki Ba다Ji-Min Kim
Keimyung Author(s)
Kim, Sang HyonSon, Chang NamKim, Ji MinBaek, Won Ki
Department
Dept. of Internal Medicine (내과학)
Dept. of Microbiology (미생물학)
Journal Title
Korean Journal of Internal Medicine.
Issued Date
2016
Keyword
Grape seed proanthocyanidin extractArthritis, experimentalToll-like receptor 4Arthritis, rheumatoid
Abstract
Background/Aims: Grape seed proanthocyanidin extract (GSPE) has been reported
to have a beneficial effect on regulating inf lammation. However, the
anti-inflammatory mechanism of GSPE remains unclear. The aim of this study
was to verify the influence of GSPE on the Toll-like receptor 4 (TLR4)-mediated
signaling pathway in the regulation of murine autoimmune arthritis.
Methods: Collagen-induced arthritis (CIA) was induced in dilute brown non-agouti
(DBA)/1J mice. The mice were treated with GSPE (0 or 100 mg/kg) intraperitoneally.
The severity of arthritis was assessed clinically, biochemically, and histologically.
Immunostaining for TLR4 was performed. The expressions of TLR4 and
downstream signaling molecules were analyzed by Western blot. The effect of
GSPE on lipopolysaccharide (LPS)-induced TLR4 activation was also evaluated
using RAW264.7 cells and fibroblast-like synoviocytes (FLSs) from patients with
rheumatoid arthritis and from those with osteoarthritis.
Results: GSPE attenuated the clinical severity of arthritis and decreased histological
damage. GSPE treatment reduced the number of TLR4-stained cells
in the synovium of mice with CIA. GSPE also downregulated the expression of
TLR4, myeloid differentiation factor 88 (MyD88) and phosphorylated IκBα synovial
protein in CIA mice. Concurrently, GSPE inhibited the nuclear translocation
of nuclear factor-κB (NF-κB) subunits (p65 and p50). LPS-induced TLR4 activation
was suppressed by GSPE in human FLS as well as in murine macrophages in
vitro.
Conclusions: Our results demonstrated that GSPE ameliorated CIA by regulating
the TLR4-MyD88-NF-κB signaling pathway.
Keimyung Author(s)(Kor)
김상현
손창남
김지민
백원기
Publisher
School of Medicine
Citation
Sang-Hyon Kim et al. (2016). Grape seed proanthocyanidin extract ameliorates murine autoimmune arthritis through regulation of TLR4/MyD88/NF-κB signaling pathway. Korean Journal of Internal Medicine., 1–10. doi: 10.3904/kjim.2016.053
Type
Article
ISSN
1226-3303
DOI
10.3904/kjim.2016.053
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33211
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.