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Microsatellite alteration in histologically normal lung tissue of patients with non-small cell lung cancer

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Author(s)
Jae Yong ParkHyo-Sung JeonSun Ha ParkTae In ParkJi-Woong SonChang Ho KimJae-Ho ParkIn San KimTae Hoon JungSu Han Jun
Keimyung Author(s)
Park, Jae Ho
Department
Dept. of Internal Medicine (내과학)
Journal Title
Lung Cancer
Issued Date
2000
Volume
30
Issue
2
Keyword
Microsatellite alterationHistologically normal lung tissueLung cancer
Abstract
Microsatellite alteration (MSA) has been observed in a fraction of non-small cell lung cancer (NSCLC). Most prior studies regarding MSA in lung cancer have usually used adjacent non-malignant lung tissues as a source of constitutional DNA. However, these normal tissues might have genetic alterations because the entire field of bronchial tree is exposed to the same carcinogenic insult. The aim of this study was to search if MSA is present in the histologically normal lung tissue of patients with NSCLC. Tumor and corresponding normal lung tissue specimens were obtained from 20 patients with NSCLC. Normal lung tissue specimens were obtained from either the opposite end of resected surgical samples or as distant from the tumor as possible. They were examined histopathologically and confirmed as normal by H-E stain. Patients’ peripheral lymphocytes were used as the source for the normal DNA. Sixteen markers on 3p and 9p (nine and seven markers, respectively) were used. MSA was detected in seven of 20 (35%) histologically normal lung tissue specimens at a frequency similar to that observed in tumor tissue (eight of 20, 40%). Five cases showed MSA in both normal lung tissue and the corresponding tumor. In these five cases, MSA in normal lung tissue was detected at the same microsatellite markers which MSA was detected in the corresponding tumor. The number and size of novel bands in normal lung tissue was identical to that in tumor tissue except in one case. In which case, the same pattern of MSA was found in both normal lung tissue and corresponding tumor tissue at two markers. However, at one marker, while one identical novel band was detected in normal lung tissue and corresponding tumor tissue, another novel band was found only in tumor tissue. In two of 12 patients whose tumor was negative for the presence of MSA, MSA was detected in normal lung tissue. These results indicate that genetic alterations are widely distributed in the lung tissue of patients with lung cancer and provide considerable support for the field cancerization theory. Screening for MSA in resected normal lung tissue might be a new method to identify patients at high risk for developing second primary lung cancers.

Keywords
Microsatellite alteration;
Histologically normal lung tissue;
Lung cancer
Keimyung Author(s)(Kor)
박재호
Publisher
School of Medicine
Citation
Jae Yong Park et al. (2000). Microsatellite alteration in histologically normal lung tissue of patients with non-small cell lung cancer. Lung Cancer, 30(2), 83–89. doi: 10.1016/S0169-5002(00)00189-6
Type
Article
ISSN
0169-5002
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S0169500200001896?via%3Dihub
DOI
10.1016/S0169-5002(00)00189-6
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33404
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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