계명대학교 의학도서관 Repository

Evidence of enhancement of malate-aspartate shuttle activity in β cells of streptozotocin-induced non—insulin-dependent diabetic rats

Metadata Downloads
Author(s)
Young-Ho AhnJae Hoon BaeWon Kyun ParkYoung-Su HongWon-Kyung HoYung E. EarmDae-Kyu Song
Keimyung Author(s)
Song, Dae KyuBae, Jae HoonPark, Won Kyun
Department
Dept. of Physiology (생리학)
Dept. of Medical Education (의학교육학)
Journal Title
Metabolism
Issued Date
2000
Volume
49
Issue
1
Abstract
Glucose-induced insulin secretion is selectively impaired in β cells from animals with non—insulin-dependent diabetes mellitus (NIDDM). This study was performed to clarify whether the malate-aspartate shuttle among the glucose metabolic pathways is intact in β cells of NIDDM rats. The insulin secretory capacity of the islets and the KATP channel activity in single β cells were measured in control and NIDDM rats injected with streptozotocin (STZ) during the neonatal period, using a radioimmunoassay and patch-clamp technique. The increase of insulin secretion induced by 11.1 mmol/L glucose or 10 mmol/L dihydroxyacetone (DHA) was significantly reduced in NIDDM islets, suggesting an impaired glycerol-phosphate shuttle. The application of glyceraldehyde (10 mmol/L) in NIDDM or control islets elicited an increase in insulin secretion, but the difference between the 2 groups was indistinguishable. On the contrary, the increase of insulin secretion and the inhibition of KATP channel activity induced by aspartate, which preferentially participates in the malate-aspartate shuttle, were significantly greater in NIDDM versus the control. However, intracellularly applied aspartate in the inside-out mode did not inhibit KATP channel activity. These findings show that malate-aspartate shuttle activity is potentiated in pancreatic β cells of NIDDM rats, suggesting the development of a compensatory mechanism for the reduced activity of the glycerol-phosphate shuttle in NIDDM.
Keimyung Author(s)(Kor)
송대규
배재훈
박원균
Publisher
School of Medicine
Citation
Young-Ho Ahn et al. (2000). Evidence of enhancement of malate-aspartate shuttle activity in β cells of streptozotocin-induced non—insulin-dependent diabetic rats. Metabolism, 49(1), 92–96. doi: 10.1016/S0026-0495(00)90838-2
Type
Article
ISSN
0026-0495
Source
https://www.sciencedirect.com/science/article/pii/S0026049500908382?via%3Dihub
DOI
10.1016/S0026-0495(00)90838-2
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33423
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Medical Education (의학교육학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
Authorize & License
  • Authorize공개
  • EmbargoForever
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.