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Microvascular cell death in spontaneously hypertensive rats during experimental inflammation

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Author(s)
KYO C. MUNFRANK A. DELANOEDWARD D. TRANGEERT W. SCHMID-SCHÖNBEIN
Keimyung Author(s)
Mun, Kyo Cheol
Department
Dept. of Biochemistry (생화학)
Journal Title
Microcirculation
Issued Date
2002
Volume
9
Issue
5
Keyword
mesenteryendotheliumleukocyteneutrophilinflammationmigrationadhesioncell deathapoptosis
Abstract
Objective: Chronic hypertension is associated with an increased risk for tissue
injury that may be mediated in part by endothelium and inflammatory cells. To
clarify a possible underlying mechanisms, we examined leukocyte migration in
the microcirculation and concomitant parenchymal cell death.
Methods: The mesentery of spontaneously hypertensive rats (SHRs) and their
normotensive controls, Wistar Kyoto (WKY) rats, was examined with digital
fluorescence microscopy after topical stimulation with an inflammatory mediator
(f-met-leu-phe, 10−8M). The migratory pathways of individual leukocytes
were traced, and at the same time cell death was detected by use of a life-death
indicator (propidium iodide) over a period of 3 hours.
Results: Both WKY and SHR had a progressively increasing number of leukocytes
migrating across the endothelium in postcapillary venules into the tissue
parenchyma. But parenchymal cell death was detected in a random pattern in
the mesentery tissue, without correlation to the migratory positions of the leukocytes.
Although mature SHR rats (about 17 weeks) exhibited the same level of
cell death as age-matched WKY rats, older WKY rats (about 30 weeks) had
significantly lower levels of cell death, whereas the SHR rats maintained the
same number of parenchymal cell death as mature animals.
Conclusions: These results suggest that in the presence of an inflammatory
mediator, the SHR may exhibit a stronger response to an inflammatory mediator
than normotensive WKY rats in a fashion that is age, but not blood pressure,
dependent. Parenchymal cell death does not correlate with migration of activated
leukocytes at the microvascular level.
Microcirculation (2002) 9, 397–405.
KEY WORDS: mesentery, endothelium, leukocyte, neutrophil, inflammation, migration,
adhesion, cell death, apoptosis.
Keimyung Author(s)(Kor)
문교철
Publisher
School of Medicine
Citation
KYO C. MUN et al. (2002). Microvascular cell death in spontaneously hypertensive rats during experimental inflammation. Microcirculation, 9(5), 397–405.
Type
Article
ISSN
1073-9688
Source
https://onlinelibrary.wiley.com/doi/abs/10.1038/sj.mn.7800152
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33427
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
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