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Anti-inflammatory effects of anthocyanins from black soybean seed coat on the keratinocytes and ischemia-reperfusion injury in rat skin flaps

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Author(s)
HYE JUNG KIMLIANJI XUKI CHURL CHANGSUNG CHUL SHINJONG IL CHUNGDAWON KANGSANG-HYON KIMJI AN HURTAE HYUN CHOISUKWHA KIMJAEHOON CHOI
Keimyung Author(s)
Kim, Sang Hyon
Department
Dept. of Internal Medicine (내과학)
Journal Title
Microsurgery
Issued Date
2012
Volume
32
Issue
7
Abstract
Ischemia-reperfusion injury is a phenomenon that occurs when tissues are subjected to ischemia for a variable period of time, and then
reperfused. Inflammatory reaction has been implicated as one of the most important mechanism of ischemia-reperfusion injury. The pur-
pose of this study was to evaluate the anti-inflammatory effects of anthocyanins from black soybean seed coat on keratinocytes in vitro
and ischemia-reperfusion injury in vivo. We investigated the inhibition, by anthocyanins, of the expression of various inflammatory genes
associated with ischemia-reperfusion injury in the tumor necrosis factor-alpha-treated (TNF-a) immortalized epidermal keratinocyte cell line
(HaCaT). We also investigated the effects of anthocyanins on the survival of skin flaps after ischemia-reperfusion injury in the rats. Accord-
ing to Western blot analysis and a luciferase activity assay, anthocyanins inhibited TNF-a-induced intercellular adhesion molecule-1 and
cyclooxygenase-2 (COX-2) levels through the NF-jB-dependent pathway. Administration of anthocyanins (50 and 100 mg/kg) significantly
improved the flap area survival in the 10-hour ischemic model from 62% to 74.5% and 83%, respectively (P 5 0.001). The related cyto-
kines in skin flap also changed as the same patter n as in vitro. Our results indicate that anthocyanins from black soybean seed coat had
anti-inflammatory effects on the HaCaT cell line and increase the survival of skin flaps through anti-inflammatory properties against ische-
mia-reperfusion injury.
Microsurgery 32:563–570, 2012.
Keimyung Author(s)(Kor)
김상현
Publisher
School of Medicine
Citation
HYE JUNG KIM et al. (2012). Anti-inflammatory effects of anthocyanins from black soybean seed coat on the keratinocytes and ischemia-reperfusion injury in rat skin flaps. Microsurgery, 32(7), 563–570. doi: 10.1002/micr.22019
Type
Article
ISSN
0738-1085
Source
http://lps3.onlinelibrary.wiley.com.proxy.dsmc.or.kr/doi/abs/10.1002/micr.22019
DOI
10.1002/micr.22019
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33428
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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