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Regulatory role of osteopontin in malignant transformation of endometrial cancer

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Author(s)
Sabarish RamachandranKun-Young KwonSo-Jin ShinSang-Hoon KwonSoon-Do ChaHyun-Gyo LeeYoung-Bin HongInsoo BaeGun-Ho LeeChi-Heum Cho
Keimyung Author(s)
Kwon, Kun YoungShin, So JinKwon, Sang HoonCha, Soon DoCho, Chi Heum
Department
Dept. of Pathology (병리학)
Dept. of Obstetrics & Gynecology (산부인과학)
Journal Title
Molecular Biology Reports
Issued Date
2013
Volume
40
Issue
5
Keyword
Endometrial cancerOsteopontinsiRNAApoptosis
Abstract
Osteopontin (OPN) involves in the tumorpromoting
or metastasis in human endometrial cancer.
Depletion of OPN gene expression in endometrial cancer
cells was significantly decreased in cell viability and the
cells undergo apoptotic cell death. The status of OPN in
THESC, RL95, Hec1A and Ishikawa cell lines were analyzed
by RT-PCR and western blot. After OPN-siRNA
transfection, mRNA and protein expression levels of OPN
were determined in Hec1A and Ishikawa cells. Cell proliferation
and cell cycle distribution were observed by
MTT and flow cytometry analysis. DNA fragmentation
assay was used to measure cell apoptosis. Cell migration
was assessed by wound healing assay. Depletion of OPN
gene expression in endometrial cancer cell lines (Hec1A
and Ishikawa cells) reproducibly changed their ability
of proliferation. Concomitant changes were seen in the
expression of OPN binding cell surface receptors, cell
cycle-regulatory genes, cell invasion and colony formation
nature of the tumor cells. Decreased colonizing potential in
the absence of OPN was reversed in the presence of
recombinant OPN. Inhibition of anchorage-independent
growth was observed in the presence of metabolic inhibitors
of the PI3K, Src and integrin signaling cascades, which
was ameliorated in the presence of exogenously added
OPN. Our result showed the role of OPN in endometrial
cancer, in particular on the malignancy-promoting aspects
of OPN that may pave way for new approaches to the
clinical management of endometrial cancer.

Keywords: Endometrial cancer Osteopontin
siRNA Apoptosis
Keimyung Author(s)(Kor)
권건영
신소진
권상훈
차순도
조치흠
Publisher
School of Medicine
Citation
Sabarish Ramachandran et al. (2013). Regulatory role of osteopontin in malignant transformation of endometrial cancer. Molecular Biology Reports, 40(5), 3623–3629. doi: 10.1007/s11033-012-2436-8
Type
Article
ISSN
0301-4851
Source
http://lps3.link.springer.com.proxy.dsmc.or.kr/article/10.1007%2Fs11033-012-2436-8
DOI
10.1007/s11033-012-2436-8
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33434
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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