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Analysis of proteome and transcriptome of tumor necrosis factor α stimulated vascular smooth muscle cells with or without alpha lipoic acid

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Affiliated Author(s)
김혜순박근규한성욱허승호이인규
Alternative Author(s)
Kim, Hye SoonPark, Keun GyuHan, Seong WookHur, Seung HoLee, In Kyu
Journal Title
Proteomics
ISSN
1615-9853
Issued Date
2004
Keyword
Alpha lipoic acidMicroarrayTumor necrosis factor aTwo-dimensional gel electrophoresisVascular smooth muscle cell
Abstract
Vascular smooth muscle cells (VSMCs) play an important role in the development and progression of atherosclerosis. Tumor necrosis factor α (TNFα), a cytokine secreted by VSMCs and macrophages in atherosclerotic lesions, regulates a variety of cellular functions of inflammatory cells and VSMCs by promoting cell growth and motility, which are critical for the initiation and progression of vascularlesions. Alpha lipoic acid (ALA), a well known antioxidant, acts as a pyruvate dehydrogenase cofactor in mitochondrial metabolism. Recently, we reported that ALA has many beneficial effects on vascular cells in atherosclerosis. The aim of the current study was to examine VSMCs, treated for 24 hours with TNFα (10 ng/mL) in the presence or absence of ALA (2 mM), for differential protein and genes expression using two-dimensional gel electrophoresis (2-DE) and DNA microarray analysis, respectively. Using 2-DE, we identified proteins whose expression changed by at least 2.5-fold after TNFα stimulation. Proteins up-regulated by TNFα that were subsequently down-regulated in the presence of ALA were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry as plasminogen activator inhibitor-2, fetal liver LKB-interacting protein, osteoblast-specific factor 2, glucosidase II, cyclin-dependent kinase 3, endoplasmin precursor and glutathione synthetase. TNFα down-regulated proteins that were up-regulated in the presence of ALA were keratin 19, eukaryotic translation elongation factor and Rho GDP dissociation inhibitor α. Gene expression analysis using DNA microarray tools confirmed the up-regulation or down-regulation of some, but not all, of the proteins observed in ALA challenged, TNFα-treated cells. This data should provide valuable information about the underlying mechanisms of atherosclerosis.
Keywords: Alpha lipoic acid / Microarray / Tumor necrosis factor a / Two-dimensional gel electrophoresis /
Vascular smooth muscle cell
Department
Dept. of Internal Medicine (내과학)
Publisher
School of Medicine
Citation
Won Gu Jang et al. (2004). Analysis of proteome and transcriptome of tumor necrosis factor α stimulated vascular smooth muscle cells with or without alpha lipoic acid. Proteomics, 4(11), 3383–3393. doi: 10.1002/pmic.200400972
Type
Article
ISSN
1615-9853
DOI
10.1002/pmic.200400972
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33455
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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