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Application of platelet-rich plasma accelerates the wound healing process in acute and chronic ulcers through rapid migration and upregulation of cyclin A and CDK4 in HaCaT cells

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Author(s)
SUNG-AE KIMHAN-WON RYUKYU-SUK LEEJAE-WE CHO
Keimyung Author(s)
Kim, Sung AeLee, Kyu SukCho, Jae We
Department
Dept. of Dermatology (피부과학)
Journal Title
Molecular Medicine Reports
Issued Date
2013
Volume
7
Issue
2
Keyword
platelet-rich plasmaskin ulcerkeratinocytescell cycles
Abstract
Application of autologous platelet-rich plasma (PRP) has been used for chronic wound healing. The aim of this study was to evaluate the effect of PRP on the wound healing processes of both acute and chronic ulcers and the underlying molecular mechanisms involved. We treated 16 patients affected by various acute and chronic ulcers with PRP. We performed molecular studies of cell proliferation, migration assays, immunoblotting and chloramphenicol acetyltransferase (CAT) assays in PRP-treated HaCaT keratinocyte cells. PRP treatment induced increased rates of cell proliferation and cell migration of HaCaT cells. In addition, the expression of cyclin A and cyclin dependent kinase (CDK) 4 proteins was markedly increased with a low concentration (0.5%) of PRP treatment in HaCaT cells. In 11 patients with chronic ulcers, including stasis ulcers, diabetic ulcers, venous leg ulcers, livedoid vasculitis, claw foot and traumatic ulcers, 9 patients showed 90-100% epithelization after 15.18 days. In 5 patients with acute ulcers, such as dehiscence, open wound and burn wound, 80-100% epithelization was achieved between 4 to 20 days. Topical application of PRP to acute and chronic skin ulcers significantly accelerated the epithelization process, likely through upregulation of the cell cycle regulatory proteins cyclin A and CDK4.
Key words: platelet-rich plasma, skin ulcer, keratinocytes, cell cycles
Keimyung Author(s)(Kor)
김성애
이규석
조재위
Publisher
School of Medicine
Citation
SUNG-AE KIM et al. (2013). Application of platelet-rich plasma accelerates the wound healing process in acute and chronic ulcers through rapid migration and upregulation of cyclin A and CDK4 in HaCaT cells. Molecular Medicine Reports, 7(2), 476–480. doi: 10.3892/mmr.2012.1230
Type
Article
ISSN
1791-2997
Source
http://lps3.www.spandidos-publications.com.proxy.dsmc.or.kr/mmr/7/2/476
DOI
10.3892/mmr.2012.1230
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33459
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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