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Carbamylated albumin stimulates microRNA-146, which is increased in human renal cell carcinoma

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Author(s)
EUNYOUNG HAJI-HYE BANGJUNG NAM SONHO-CHAN CHOKYO-CHUL MUN
Keimyung Author(s)
Ha, Eun YoungMun, Kyo CheolCho, Ho Chan
Department
Dept. of Biochemistry (생화학)
Dept. of Internal Medicine (내과학)
Kidney Institute (신장연구소)
Journal Title
Molecular Medicine Reports
Issued Date
2010
Volume
3
Issue
2
Keyword
carbamylationalbuminmicroRNArenal cell carcinoma
Abstract
Carbamylation is a post-translational modification, the pathophysiological consequences of which remain poorly understood. MicroRNAs (miRNAs) are endogenous non-coding small ribonucleic acids that have emerged as one of the central players in gene expression regulation. This study was designed to determine the effect of carbamylated albumin (cAlb) on the expression of miRNAs. Albumin was carbamylated, and the extent of carbamylation was monitored using trinitrobenzenesulphonic acid. Albumin or cAlb were added to rat mesangial cells (RMCs), and RNA was extracted. miRNA microarray analysis was performed. The expression of microRNA-146a (miR-146a) and microRNA-146b (miR-146b) was analyzed by real-time RT-PCR. Of 365 miRNAs analyzed, the expression of miR-146a/b was found to be markedly induced by cAlb (miR-146a, 12.75-fold increase; miR-146b, 5.88-fold increase). Real-time RT-PCR analysis confirmed the increased levels of miR-146a/b by cAlb (p<0.05). It was also found that expression levels of miR-146a/b were increased in renal cell carcinoma tumor tissues compared to corresponding non-tumor tissues (p<0.05). Our data suggest that cAlb stimulates miR-146a/b in RMCs, the levels of which are increased in renal cell carcinoma. Further studies on the function of cAlb may provide new insights into the pathophysiology of renal cell carcinoma.
Key words: carbamylation, albumin, microRNA, renal cell carcinoma
Keimyung Author(s)(Kor)
하은영
문교철
조호찬
Publisher
School of Medicine
Citation
EUNYOUNG HA et al. (2010). Carbamylated albumin stimulates microRNA-146, which is increased in human renal cell carcinoma. Molecular Medicine Reports, 3(2), 275–279. doi: 10.3892/mmr_00000251
Type
Article
ISSN
1791-2997
Source
http://lps3.www.spandidos-publications.com.proxy.dsmc.or.kr/mmr/3/2/275
DOI
10.3892/mmr_00000251
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33460
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
3. Research Institutues (연구소) > Kidney Institute (신장연구소)
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