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Carbamylated low-density lipoprotein attenuates glucose uptake via a nitric oxide-mediated pathway in rat L6 skeletal muscle cells

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Author(s)
HYE-JUNG CHOIKYOUNG JAE LEEEUN AH HWANGKYO-CHEOL MUNEUNYOUNG HA
Keimyung Author(s)
Mun, Kyo CheolHa, Eun YoungLee, Kyung JaeHwang, Eun Ah
Department
Dept. of Biochemistry (생화학)
Dept. of Orthopedic Surgery (정형외과학)
Dept. of Internal Medicine (내과학)
Kidney Institute (신장연구소)
Journal Title
Molecular Medicine Reports
Issued Date
2015
Volume
12
Issue
1
Keyword
carbamylated low-density lipoproteininducible nitric oxide synthaseglucose uptakeskeletal muscle cells
Abstract
Carbamylation is a cyanate‑mediated posttranslational modification. We previously reported that carbamylated low‑density lipoprotein (cLDL) increases reactive oxygen species and apoptosis via a lectin‑like oxidized LDL receptor mediated pathway in human umbilical vein endothelial cells. A recent study reported an association between cLDL and type 2 diabetes mellitus (T2DM). In the current study, the effects of cLDL on glucose transport were explored in skeletal muscle cells. The effect of cLDL on glucose uptake, glucose transporter 4 (GLUT4) translocation, and signaling pathway were examined in cultured rat L6 muscle cells using 2‑deoxyglucose uptake, immunofluorescence staining and western blot analysis. The quantity of nitric oxide (NO) was evaluated by the Griess reaction. The effect of native LDL (nLDL) from patients with chronic renal failure (CRF‑nLDL) on glucose uptake was also determined. It was observed that cLDL significantly attenuated glucose uptake and GLUT4 translocation to the membrane, which was mediated via the increase in inducible nitric oxide synthase (iNOS)‑induced NO production. Tyrosine nitration of the insulin receptor substrate‑1 (IRS‑1) was increased. It was demonstrated that CRF‑nLDL markedly reduced glucose uptake compared with nLDL from healthy subjects. Collectively, these findings indicate that cLDL, alone, attenuates glucose uptake via NO-mediated tyrosine nitration of IRS‑1 in L6 rat muscle cells and suggests the possibility that cLDL is involved in the pathogenesis of T2DM.
Keimyung Author(s)(Kor)
문교철
하은영
이경재
황은아
Publisher
School of Medicine
Citation
HYE-JUNG CHOI et al. (2015). Carbamylated low-density lipoprotein attenuates glucose uptake via a nitric oxide-mediated pathway in rat L6 skeletal muscle cells. Molecular Medicine Reports, 12(1), 1342–1346. doi: 10.3892/mmr.2015.3481
Type
Article
ISSN
1791-2997
Source
http://lps3.www.spandidos-publications.com.proxy.dsmc.or.kr/mmr/12/1/1342
DOI
10.3892/mmr.2015.3481
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33461
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학)
3. Research Institutues (연구소) > Kidney Institute (신장연구소)
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