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Cyanate attenuates insulin secretion in cultured pancreatic β cells

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Author(s)
EUNYOUNG HA
Keimyung Author(s)
Ha, Eun Young
Department
Dept. of Biochemistry (생화학)
Journal Title
Molecular Medicine Reports
Issued Date
2012
Volume
5
Issue
6
Keyword
cyanateinsulinreactive oxygen speciespancreatic β cells
Abstract
The vast majority of long-term complications in transplanted patients are associated with cardiovascular disease. Previously, an alternative and dominant mechanism for cyanate formation in atherosclerotic lesions has been discovered. This study was designed to determine the effect of cyanate on insulin secretion in cultured pancreatic β cells (INS-1 cells). The cytotoxicity of cyanate was determined by 3-(4,5-Desethyithiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Insulin secretion was measured by ELISA in cyanate-treated INS-1 cells. Reactive oxygen species (ROS) generation was also determined by measuring the fluorescent oxidized product of 2,7-dichlorefluorescein in cyanate-treated INS-1 cells. FACS analysis was carried out to determine the effect of cyanate on the apoptosis of INS-1 cells. Firstly, we found that cyanate, within concentration ranges in which no cytotoxic effect was observed (0.01, 0.1 and 1.0 mM), decreased insulin secretion dose-dependently in both non-glucose-stimulated and glucose-stimulated INS-1 cells. Cyanate at a 1.0 mM concentration inhibited insulin secretion by more than 50% in non-glucose-stimulated cells and glucose (5 and 10 mM)-stimulated cells. Cyanate, however, did not affect ROS generation. Furthermore, no pro- or anti-apoptotic effect was observed in cyanate-treated INS-1 cells. The results in this study suggest the possible inhibitory effect of cyanate on insulin secretion in INS-1 pancreatic β cells. The inhibitory effect was not mediated either by ROS generation or by apoptosis. Further studies to determine the underlying mechanisms will be of benefit.
Keimyung Author(s)(Kor)
하은영
Publisher
School of Medicine
Citation
EUNYOUNG HA. (2012). Cyanate attenuates insulin secretion in cultured pancreatic β cells. Molecular Medicine Reports, 5(6), 1461–1464. doi: 10.3892/mmr.2012.837
Type
Article
ISSN
1791-2997
Source
http://lps3.www.spandidos-publications.com.proxy.dsmc.or.kr/mmr/5/6/1461
DOI
10.3892/mmr.2012.837
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33462
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
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