계명대학교 의학도서관 Repository

Apoptosis induction of human leukemia U937 cells by 7,8-dihydroxyflavone hydrate through modulation of the Bcl-2 family of proteins and the MAPKs signaling pathway

Metadata Downloads
Author(s)
Hye Young ParkGi-Young KimTaeg Kyu KwonHye Jin HwangNam Deuk KimYung Hyun ChoiYoung Hyun Yoo
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Mutation Research-Genetic Toxicology and Environmetal
Issued Date
2013
Volume
751
Issue
2
Keyword
U937 cells7,8-Dihydroxyflavone hydrateApoptosisBcl-2MAPKs
Abstract
The present study investigated possible mechanisms of apoptosis induction of U937 human leukemic cells by 7,8-dihydroxyflavone hydrate (7,8-DHF), a member of the flavonoid family and a recently identified tyrosine kinase receptor B (TrkB) agonist. 7,8-DHF treatment of U937 cells resulted in inhibition of growth and induction of apoptosis as measured by MTT assay, fluorescence microscopy, DNA fragmentation, and flow cytometry analysis. 7,8-DHF-induced apoptosis in U937 cells was correlated with the up-regulation of death receptor related protein levels and down-regulation of anti-apoptotic IAP family proteins. The increase in apoptosis was also associated with proteolytic activation of caspases, Bid cleavage, insertion of pro-apoptotic Bax into the mitochondria and release of cytochrome c from mitochondria to cytosol. Furthermore, it was found that Bcl-2 overexpression markedly protected U937 cells from 7,8-DHF-induced apoptosis by restoring activation of caspases. In addition, 7,8-DHF treatment effectively activated the mitogen-activated protein kinases (MAPK), and inhibitors of extracellular-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK), but not p38 MAPK, which significantly reduced 7,8-DHF-induced apoptosis. Taken together, our results indicate that the JNK and ERK pathways, and modulation of Bcl-2 family proteins were key regulators of apoptosis in response to 7,8-DHF in U937 cells.
Keywords: U937 cells; 7,8-Dihydroxyflavone hydrate; Apoptosis; Bcl-2; MAPKs
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
Hye Young Park et al. (2013). Apoptosis induction of human leukemia U937 cells by 7,8-dihydroxyflavone hydrate through modulation of the Bcl-2 family of proteins and the MAPKs signaling pathway. Mutation Research-Genetic Toxicology and Environmetal, 751(2), 101–108. doi: 10.1016/j.mrgentox.2012.12.002
Type
Article
ISSN
1383-5718
Source
https://www.sciencedirect.com/science/article/pii/S1383571812003580?via%3Dihub
DOI
10.1016/j.mrgentox.2012.12.002
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33465
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.