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Role of (−)-epigallocatechin-3-gallate in cell viability, lipogenesis, and retinol-binding protein 4 expression in adipocytes

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Author(s)
Hye-Young SungChae-Geun HongYoung-Sung SuhHo-Chan ChoJae-Hyung ParkJae-Hoon BaeWon-Kyun ParkJin HanDae-Kyu Song
Keimyung Author(s)
Suh, Young SungCho, Ho ChanPark, Jae HyungBae, Jae HoonSong, Dae KyuPark, Won Kyun
Department
Dept. of Family Medicine (가정의학)
Dept. of Internal Medicine (내과학)
Dept. of Physiology (생리학)
Dept. of Medical Education (의학교육학)
Journal Title
Naunyn-Schmiedeberg's Archives of Pharmacology
Issued Date
2010
Volume
382
Issue
4
Keyword
Epigallocatechin gallateCatechinPlasma retinol-binding proteinAdipocyteGlucose uptakeReactive oxygen species
Abstract
(−)-Epigallocatechin-3-gallate (EGCG), a bioactive
compound of green tea, is known to combat obesity by
reducing the viability and lipid accumulation of adipocytes. In
this study, we evaluated the mechanism and clinical relevance
on those actions of EGCG. We measured the viability of
3T3-L1 preadipocytes and adipocytes by the 3-(4, 5-
dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide assay.
Lipid accumulation was measured by Oil Red O staining.
Intracellular accumulation of reactive oxygen species (ROS)
was determined using a flow cytometer. Cellular glucose
uptake was determined with 2-deoxy-[3H]-glucose. The
protein levels of peroxisome proliferator-activated receptor
(PPAR)-γ and adiponectin in 3T3-L1 adipocytes, as well as
the protein level and secretion of plasma retinol-binding
protein (RBP4) in human adipocytes, were measured by
western blot. EGCG at concentrations higher than 10 μM
induced ROS generation and decreased the viability and lipid
accumulation of adipocytes. It also decreased the expression
of PPAR-γ and adiponectin. At concentrations readily
achievable in human plasma via green tea intake
(≤10 μM), EGCG inhibited cellular glucose uptake and
enhanced the expression and secretion of RBP4 in adipocytes.
Pharmacological doses of EGCG showed cytotoxic
effects in preadipocytes and adipocytes. EGCG-mediated
glucose uptake inhibition in adipocytes may be clinically
relevant and is probably linked to the increase in the
expression and secretion of RBP4. Because secreted RBP4
from adipocytes inhibits muscular glucose uptake and
enhance hepatic glucose output, the systemic effect of
EGCG associated with its effect on RBP4 secretion should
be further determined, as it may negatively regulate wholebody
insulin sensitivity, contrary to general belief.
Keywords: Epigallocatechin gallate . Catechin . Plasma retinol-binding protein . Adipocyte . Glucose uptake . Reactive oxygen species
Keimyung Author(s)(Kor)
서영성
조호찬
박재형
배재훈
송대규
박원균
Publisher
School of Medicine
Citation
Hye-Young Sung et al. (2010). Role of (−)-epigallocatechin-3-gallate in cell viability, lipogenesis, and retinol-binding protein 4 expression in adipocytes. Naunyn-Schmiedeberg’s Archives of Pharmacology, 382(4), 303–310. doi: 10.1007/s00210-010-0547-0
Type
Article
ISSN
0028-1298
Source
https://link.springer.com/article/10.1007%2Fs00210-010-0547-0
DOI
10.1007/s00210-010-0547-0
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33476
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Family Medicine (가정의학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Medical Education (의학교육학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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