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Grape seed proanthocyanidin extract inhibits interleukin-17-induced interleukin-6 production via MAPK pathway in human pulmonary epithelial cells

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Author(s)
Hyeongseok KimJin Young KimHong Suk SongKeon Uk ParkKyo-Chul MunEunyoung Ha
Keimyung Author(s)
Mun, Kyo CheolHa, Eun YoungKim, Jin YoungSong, Hong SukPark, Keon Uk
Department
Dept. of Biochemistry (생화학)
Dept. of Internal Medicine (내과학)
Journal Title
Naunyn-Schmiedeberg's Archives of Pharmacology
Issued Date
2011
Volume
383
Issue
6
Keyword
GSPEIL-17Pulmonary epithelial cellsInflammationIL-6
Abstract
This study was aimed to investigate the effect of
grape seed proanthocyanidin extract (GSPE) on interleukin-
17 (IL-17)-induced interleukin-6 (IL-6) production in A549
human pulmonary epithelial cells. Cells were treated with
IL-17 (10 ng/ml) or GSPE (50 μg/ml), or both. The effects
of GSPE on cell viability and apoptosis were evaluated.
The mRNA and protein levels of IL-6 were determined by
reverse transcription-polymerase chain reaction and
enzyme-linked immunosorbent assay, respectively. Expressions
of inhibitory factor κB-α (IκB-α) and ERK 1/2, p38,
and JNK mitogen-activated protein kinase (MAPK) were
examined by Western blot analysis. GSPE significantly
inhibited both GSPE treatment only and IL-17-induced
mRNA expressions and protein productions of IL-6 in
pulmonary epithelial cells (p<0.01). GSPE decreased the
IL-17-induced phosphorylation of IκB-α. The IL-17 stimulated
ERK 1/2, p38, and JNK MAPK activities and GSPE
decreased IL-17-stimulated ERK 1/2, p38, and JNK MAPK
activities. GSPE also attenuated TNF-α and IL-1β-induced
IL-6 productions (p<0.05). Our results show that GSPE
may inhibit IL-17-stimulated IL-6 productions in human
pulmonary epithelial cells by inhibiting MAPK and nuclear
factor-κB-mediated signaling pathway.
Keywords: GSPE . IL-17 . Pulmonary epithelial cells . Inflammation . IL-6
Keimyung Author(s)(Kor)
문교철
하은영
김진영
송홍석
박건욱
Publisher
School of Medicine
Citation
Hyeongseok Kim et al. (2011). Grape seed proanthocyanidin extract inhibits interleukin-17-induced interleukin-6 production via MAPK pathway in human pulmonary epithelial cells. Naunyn-Schmiedeberg’s Archives of Pharmacology, 383(6), 555–562. doi: 10.1007/s00210-011-0633-y
Type
Article
ISSN
0028-1298
Source
https://link.springer.com/article/10.1007%2Fs00210-011-0633-y
DOI
10.1007/s00210-011-0633-y
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33477
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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