Grape seed proanthocyanidin extract inhibits interleukin-17-induced interleukin-6 production via MAPK pathway in human pulmonary epithelial cells
- Author(s)
- Hyeongseok Kim; Jin Young Kim; Hong Suk Song; Keon Uk Park; Kyo-Chul Mun; Eunyoung Ha
- Keimyung Author(s)
- Mun, Kyo Cheol; Ha, Eun Young; Kim, Jin Young; Song, Hong Suk; Park, Keon Uk
- Department
- Dept. of Biochemistry (생화학)
Dept. of Internal Medicine (내과학)
- Journal Title
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Issued Date
- 2011
- Volume
- 383
- Issue
- 6
- Keyword
- GSPE; IL-17; Pulmonary epithelial cells; Inflammation; IL-6
- Abstract
- This study was aimed to investigate the effect of
grape seed proanthocyanidin extract (GSPE) on interleukin-
17 (IL-17)-induced interleukin-6 (IL-6) production in A549
human pulmonary epithelial cells. Cells were treated with
IL-17 (10 ng/ml) or GSPE (50 μg/ml), or both. The effects
of GSPE on cell viability and apoptosis were evaluated.
The mRNA and protein levels of IL-6 were determined by
reverse transcription-polymerase chain reaction and
enzyme-linked immunosorbent assay, respectively. Expressions
of inhibitory factor κB-α (IκB-α) and ERK 1/2, p38,
and JNK mitogen-activated protein kinase (MAPK) were
examined by Western blot analysis. GSPE significantly
inhibited both GSPE treatment only and IL-17-induced
mRNA expressions and protein productions of IL-6 in
pulmonary epithelial cells (p<0.01). GSPE decreased the
IL-17-induced phosphorylation of IκB-α. The IL-17 stimulated
ERK 1/2, p38, and JNK MAPK activities and GSPE
decreased IL-17-stimulated ERK 1/2, p38, and JNK MAPK
activities. GSPE also attenuated TNF-α and IL-1β-induced
IL-6 productions (p<0.05). Our results show that GSPE
may inhibit IL-17-stimulated IL-6 productions in human
pulmonary epithelial cells by inhibiting MAPK and nuclear
factor-κB-mediated signaling pathway.
Keywords: GSPE . IL-17 . Pulmonary epithelial cells . Inflammation . IL-6
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