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Human neural stem cells: electrophysiological properties of voltage-gated ion channels

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Author(s)
T. ChoJ. H. BaeH. B. ChoiS. S. KimJ. G. McLarnonH. Suh-KimS. U. KimC. K. Min
Keimyung Author(s)
Bae, Jae Hoon
Department
Dept. of Physiology (생리학)
Journal Title
Neuroreport
Issued Date
2002
Volume
13
Issue
11
Keyword
Human neural stem cellsNeuroDPotassium currentsSodium currentsPatch-clamp
Abstract
We have characterized the pro¢le ofmembrane currents in an immortalized
human neural stem cell line, HB1.F3 cells, using wholecell
patch clamp technique.Human neural stemcell line generated
from primary cell cultures of embryonic human telencephalon
using a replication-incompetent retroviral vector containing vmyc
expresses nestin, a cell type-speci¢c marker for neural stem
cells.The human neural stemcells expressed both outward and inward
Kþ currents with no evidence for Naþ currents.The density
of the outward, delayed rectifying type Kþ current was
1.870.015 nA/pF, and that of the inwardly rectifying Kþ current
was 0.3770.012 nA/pF (at 30mMof [Kþ]o). In order to induce neuronal
di¡erentiation of the neural stem cells, a full-length coding
region of NeuroD, a neurogenic transcription factor, was transfected
into HB1.F3 cells. Introduction of NeuroDi nduced expression
of Naþ currents with the current density of 0.04270.011nA/
pF. The presence of two types of Kþ currents and expression of
Naþ currents induced byNeuroDap pear to re£ect the characteristic
physiological features of human neural stemcells.NeuroReport
13:1447^1452 c 2002 LippincottWilliams &Wilkins.
Key words:Human neural stemcells; NeuroD; Potassiumcurrents; Sodiumcurrents; Patch-clamp
Keimyung Author(s)(Kor)
배재훈
Publisher
School of Medicine
Citation
T. Cho et al. (2002). Human neural stem cells: electrophysiological properties of voltage-gated ion channels. Neuroreport, 13(11), 1447–1452.
Type
Article
ISSN
0959-4965
Source
https://insights.ovid.com/pubmed?pmid=12167771
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33535
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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