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Fluoxetine increases the nitric oxide production via nuclear factor kappa B-mediated pathway in BV2 murine microglial cells

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Author(s)
Eunyoung HaKyung Hee JungBong-Keun ChoeJae-Hoon BaeDong-Hoon ShinSung-Vin YimHyung Hwan Baik
Keimyung Author(s)
Bae, Jae HoonShin, Dong HoonHa, Eun Young
Department
Dept. of Physiology (생리학)
Dept. of Preventive Medicine (예방의학)
Dept. of Biochemistry (생화학)
Institute for Cancer Research (암연구소)
Journal Title
Neuroscience Letters
Issued Date
2006
Volume
397
Issue
3
Keyword
FluoxetineNitric oxideNitric oxide synthase 2NFκBp38 MAPK
Abstract
A body of recent evidence implicates that antidepressants affect the inflammatory response and immune system. The present study is focused on the effects of the most widely used antidepressant agent, fluoxetine on the production of nitric oxide (NO) in BV2 microglial cells. In this study, we observed interesting result that NO production was increased by fluoxetine. The mRNA level of nitric oxide synthase (iNos, Nos2) by RT-PCR was also stimulated by fluoxetine. We next conducted electophoretic mobility shift assay (EMSA) to determine the DNA binding activity of nuclear factor kappa B (Nfκb), an important upstream modulator for Nos2 expression, to find that fluoxetine increased DNA binding activity of Nfκb. By Western blot analysis, phosphorylation levels of p38 mitogen-activated protein kinase (p38 Mapk, Mapk14) and extracellular signal-related kinase (Erk)1/2 Mapk, upstream signaling mediators of Nfκb were found to be increased by fluoxetine. In addition, the mRNA expressions of other proinflammatory cytokines, interleukin 6 (Il6) and tumor necrosis factor α (Tnfα) were examined. The expressions of both Il6 and Tnfα by fluoxetine treatment were similar to those of Nos2 and Nfκb. Taken together, our results show that fluoxetine stimulates NO production via Nfκb-mediated pathway in BV2 cells.

Keywords
Fluoxetine;
Nitric oxide;
Nitric oxide synthase 2;
NFκB;
p38 MAPK
Keimyung Author(s)(Kor)
배재훈
신동훈
하은영
Publisher
School of Medicine
Citation
Eunyoung Ha et al. (2006). Fluoxetine increases the nitric oxide production via nuclear factor kappa B-mediated pathway in BV2 murine microglial cells. Neuroscience Letters, 397(3), 185–189. doi: 10.1016/j.neulet.2005.12.022
Type
Article
ISSN
0304-3940
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S030439400501414X?via%3Dihub
DOI
10.1016/j.neulet.2005.12.022
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33536
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. School of Medicine (의과대학) > Dept. of Preventive Medicine (예방의학)
3. Research Institutues (연구소) > Institute for Cancer Research (암연구소)
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