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Increased aquaporin-1 expression in choroid plexus epithelium after systemic hyponatremia

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Author(s)
Yongsuk MoonSungwon J. HongDonghoon ShinYongwook Jung
Keimyung Author(s)
Shin, Dong Hoon
Department
Dept. of Preventive Medicine (예방의학)
Journal Title
Neuroscience Letters
Issued Date
2006
Volume
395
Issue
1
Keyword
Aquaporin-1Systemic hyponatremiaChoroid plexusApoptosisDentate gyrus
Abstract
The expression of aquaporin-1 (AQP1) protein, a water channel, in the choroid plexus epithelium was examined in the rat to determine the role of AQP1 in the pathophysiology of systemic hyponatremia. Systemic hyponatremia was produced by coadministration of 30 ml hypotonic dextrose solution (140 mM) intraperitoneally and 3 μg 1-deamino-8-d-arginine vasopressin (dDAVP) subcutaneously. This hydration protocol produced severe systemic hyponatremia: 2 h after drug administration, normonatremic serum osmolarity and Na+ concentration (296 ± 5 mOsm/L and 140 ± 5 mM) were reduced to 252 ± 5 mOsm/L and 117 ± 2 mM, respectively. AQP1 expression in the third ventricle choroid plexus epithelium was determined by using immunohistochemistry and quantitative immunoblot analysis. After 2 h of systemic hyponatremia, AQP1 expression was significantly increased by 28.2 ± 2.7% (n = 6, P < 0.05), whereas it was attenuated to 15.7 ± 2.2% (n = 6, P < 0.05) after 6 h of systemic hyponatremia. In addition, TUNEL staining revealed apoptotic granule cells in the dentate gyrus (DG) adjacent to the third ventricle with a peak measured 2 h after the start of the systemic hyponatremia protocol, while virtually no apoptotic granule cells left 6 h after the start of the hyponatremic treatment. These findings suggest that AQP1-mediated excessive water influx into the brain through the blood-cerebrospinal fluid barrier during systemic hyponatremia may elevate the intracranial pressure and result in the apoptotic brain cell death. Thus, AQP1 in the choroid plexus may play an important role in the pathogenesis of systemic hyponatremia.

Keywords
Aquaporin-1;
Systemic hyponatremia;
Choroid plexus;
Apoptosis;
Dentate gyrus
Keimyung Author(s)(Kor)
신동훈
Publisher
School of Medicine
Citation
Yongsuk Moon et al. (2006). Increased aquaporin-1 expression in choroid plexus epithelium after systemic hyponatremia. Neuroscience Letters, 395(1), 1–6. doi: 10.1016/j.neulet.2005.10.060
Type
Article
ISSN
0304-3940
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S030439400501222X?via%3Dihub
DOI
10.1016/j.neulet.2005.10.060
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33549
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Preventive Medicine (예방의학)
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