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Altered expression of sodium transporters in ischemic penumbra after focal cerebral ischemia in rats

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Author(s)
Yong-Wook JungIn-Jang ChoiTae-Hwan Kwon
Keimyung Author(s)
Choi, In Jang
Department
Dept. of Anatomy (해부학)
Journal Title
Neuroscience Research
Issued Date
2007
Volume
59
Issue
2
Keyword
Brain ischemiaBrain edemaIntracellular acidosisIschemic penumbraSodium transportersTargeted proteomics
Abstract
This study was aimed to examine whether the changes of protein expression of sodium transporters in the ischemic penumbra are associated with the pathogenesis of ischemia-induced brain edema and/or brain cell injury. An experimental model of cerebral ischemia was made by permanent middle cerebral artery occlusion (pMCAO) in rats and the changes of protein expression of sodium transporters in the ischemic penumbra were examined by immunoblotting. Extensive infarction was observed in the frontal and parietal cortical and subcortical areas at 3 and 6 h after pMCAO. Immunoblotting analyses revealed significantly increased expressions of electrogenic NBC (241 ± 11% at 3 h and 154 ± 9% at 6 h, P < 0.05) and NHE1 (144 ± 3% at 3 h and 170 ± 9% at 6 h, P < 0.05), compared with sham-operated controls. In contrast, Na–K-ATPase expression (78 ± 6% at 3 h and 85 ± 3% at 6 h, P < 0.05) was significantly decreased. The expression of NCX1 was unchanged at 3 h, but was significantly increased at 6 h (141 ± 3%, P < 0.05). In addition, the expressions of neuronal (NeuN) and astroglial cell (GFAP) proteins were decreased, whereas the expression of oligodendrocyte protein (CNPase) was unchanged. Taken together, the selectively increased expressions of NHE1, electrogenic NBC, and NCX1 and decreased expression of Na–K-ATPase in the ischemic penumbra are likely to contribute to the secondary brain cell damages presumably through intracellular Na+ accumulation, cell swelling, and intracellular Ca2+ overload.

Keywords
Brain ischemia;
Brain edema;
Intracellular acidosis;
Ischemic penumbra;
Sodium transporters;
Targeted proteomics
Keimyung Author(s)(Kor)
최인장
Publisher
School of Medicine
Citation
Yong-Wook Jung et al. (2007). Altered expression of sodium transporters in ischemic penumbra after focal cerebral ischemia in rats. Neuroscience Research, 59(2), 152–159. doi: 10.1016/j.neures.2007.06.1470
Type
Article
ISSN
0168-0102
Source
https://www.sciencedirect.com/science/article/pii/S0168010207016628?via%3Dihub
DOI
10.1016/j.neures.2007.06.1470
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33552
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
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