Changes in Glucose Transporters, Gluconeogenesis, and Circadian Clock after Duodenal-Jejunal Bypass Surgery
- Author(s)
- Mikyung Kim; Young Gil Son; Yu Na Kang; Tae Kyung Ha; Eunyoung Ha
- Keimyung Author(s)
- Ha, Eun Young; Kim, Mi Kyung; Son, Young Gil; Kang, Yu Na
- Department
- Dept. of Biochemistry (생화학)
Dept. of Internal Medicine (내과학)
Dept. of Surgery (외과학)
Dept. of Pathology (병리학)
- Journal Title
- Obesity Surgery
- Issued Date
- 2015
- Volume
- 25
- Issue
- 4
- Keyword
- Circadian clock; Gluconeogenesis; Glucose transporters; Duodenal–Jejunal Bypass
- Abstract
- Background Bariatric surgery improves obesity and ameliorates
glucose tolerance. This study was conducted to evaluate
circadian clocks, gluconeogenesis, and glucose transport
changes in hepatic and intestinal tissues after duodenal–jejunal
bypass (DJB) surgery in a rat model.
Methods Twenty-five rats were randomly assigned to either
sham group (10 rats) or DJB group (15 rats). Food intake,
body weight, blood glucose, and serum insulin levels were
measured. Quantitative RT-PCR, immunoblot, and immunohistochemistry
were used to analyze genes and proteins in the
liver and intestine.
Results Food intake and body weight were not different between
sham and DJB groups. Blood glucose level was significantly
lower in the DJB group comparedwith that in the sham
group. Although not significant, serum insulin level showed
an increased tendency in DJB group. DJB induced marked
expressions of glucose transporter-2 (GLUT2) in the liver and
GLUT2 and sodium-dependent glucose transporter-1
(SGLT1) in the intestine. Gluconeogenic enzymes
[phosphoenolpyruvate carboxykinase-1 (Pck1) and glucose-
6-phosphatase (G6Pase)] decreased in the liver and increased
in the intestine of the DJB group. Circadian transcription
factor cryptochrome-1 (Cry1) increased in the liver and decreased
in the intestine of the DJB group. Another circadian
transcription factor period-2 (Per2) also increased in the liver
and decreased in the intestine of the DJB group.
Conclusion In conclusion, this study suggests the possibility
that Cry1 and Per2may mediate decreased gluconeogenesis in
the liver and increased gluconeogenesis in the intestine of the
DJB group.
Keywords Circadian clock . Gluconeogenesis .
Glucose transporters . Duodenal–Jejunal Bypass
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