Changes in Glucose Metabolism in Vertical Sleeve Gastrectomy
- Author(s)
- Yunmee Lho; Carel W. le Roux; Hyeon Soo Park; Gon Sup Kim; Jeeyoun Jung; Geum-Sook Hwang; Youn Kyoung Seo; Tae Kyung Ha; Eunyoung Ha
- Keimyung Author(s)
- Ha, Eun Young
- Department
- Dept. of Biochemistry (생화학)
- Journal Title
- Obesity Surgery
- Issued Date
- 2015
- Volume
- 25
- Issue
- 11
- Keyword
- Vertical sleeve gastrectomy; Proteome; Metabolome; Gluconeogenesis
- Abstract
- Background We evaluated metabolic changes after vertical
sleeve gastrectomy (VSG) surgery in a rat model using proteomics
and metabolomic profiling in liver and serum.
Methods Rats were randomly divided into two groups: sham
(n=10) and VSG (n=12). Food intake, body weight, blood
glucose, insulin, and thyroid hormone levels were measured.
Two-dimensional electrophoresis, nuclear resonance
spectroscopy, mass spectroscopy, immunofluorescence, and
immunoblot analyses were used to determine and validate
changes in metabolites and proteins in liver tissue and serum
samples.
Results Food intake and body weight decreased after VSG
group (p<0.05 and p<0.05, respectively). Random blood glucose
(sham, 183.3±5.6 mg/dL; VSG, 138.5±3.7 mg/dL) decreased
while random insulin (sham, 0.45±0.16 μg/L; VSG,
1.05±0.18 μg/L) increased after VSG (p<0.05 and p<0.01,
respectively). We found that expressions of gluconeogenic
enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-
6-phosphatase) and concentrations of pyruvate and malate
decreased while lactate, NADH, NADPH, glucose, and
AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine
(T3) and free thyroxine (fT4), decreased after
VSG.
Conclusion This study proves that VSG suppresses hepatic
glucose production.
Keywords Vertical sleeve gastrectomy . Proteome .
Metabolome . Gluconeogenesis
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