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Rapid blockade of telomerase activity and tumor cell growth by the DPL lipofection of ribbon antisense to hTR

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Author(s)
Arun K BajpaiJeong-Hoh ParkIk-Jae MoonHyungu KangYun-Han LeeKyung-Oh DohSeong-Il SuhByeong-Churl ChangJong-Gu Park
Keimyung Author(s)
Suh, Seong IlJang, Byeong ChurlPark, Jong Gu
Department
Dept. of Microbiology (미생물학)
Dept. of Molecular Medicine (분자의학)
Institute for Medical Science (의과학연구소)
Journal Title
Oncogene
Issued Date
2005
Volume
24
Issue
43
Keyword
telomerasehTRribbon antisenseDPL transfection
Abstract
Ribbon antisense (RiAS) to the hTR RNA, a component
of the telomerase complex, was employed to inhibit
telomerase activity and cancer cell growth. The antisense
molecule, hTR-RiAS, combined with enhanced cellular
uptake was shown to effectively inhibit telomerase activity
and cause rapid cell death in various cancer cell lines.
When cancer cells were treated with hTR-RiAS, the level
of hTR RNA was reduced by more than 90% accompanied
with reduction in telomerase activity. When
checked for cancer cell viability, cancer cell lines treated
with hTR-RiAS using DNAþPeptideþLipid complex
showed 70–80% growth inhibition in 3 days. The reduced
cell viability was due to apoptosis as the percentage of
cells exhibiting the sub-G0 arrest and DNA fragmentation
increased after antisense treatment. Further, when subcutaneous
tumors of a colon cancer cell line (SW480) were
treated intratumorally with hTR-RiAS, tumor growth was
markedly suppressed with almost total ablation of hTR
RNA in the tumor tissue. Cells in the tumor tissue were
also found to undergo apoptosis after hTR-RiAS treatment.
These results suggest that hTR-RiAS is an effective
anticancer reagent, with a potential for broad efficacy to
diverse malignant tumors.
Oncogene (2005) 24, 6492–6501. doi:10.1038/sj.onc.1208731;
published online 15 August 2005
Keywords: telomerase; hTR; ribbon antisense; DPL
transfection
Keimyung Author(s)(Kor)
서성일
장병철
박종구
Publisher
School of Medicine
Citation
Arun K Bajpai et al. (2005). Rapid blockade of telomerase activity and tumor cell growth by the DPL lipofection of ribbon antisense to hTR. Oncogene, 24(43), 6492–6501. doi: 10.1038/sj.onc.1208731
Type
Article
ISSN
0950-9232
Source
http://www.nature.com/articles/1208731
DOI
10.1038/sj.onc.1208731
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33596
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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