계명대학교 의학도서관 Repository

RU486, a glucocorticoid receptor antagonist, induces apoptosis in U937 human lymphoma cells through reduction in mitochondrial membrane potential and activation of p38 MAPK

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Author(s)
JI HOON JANGSEON MIN WOOHEE JUNG UMEUN JUNG PARKKYOUNG-JIN MINTAE-JIN LEESANG HYUN KIMYUNG HYUN CHOITAEG KYU KWON
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Oncology Reports
Issued Date
2013
Volume
30
Issue
1
Keyword
RU486apoptosiscaspase-3p38 MAPKmitochondrial membrane potential
Abstract
RU486 (mifepristone) exerts an anticancer effect on cancer cells via induction of apoptosis. However, the molecular mechanisms are not fully understood. Here, we investigated the effect of RU486 on the apoptosis of U937 human leukemia cells. RU486 markedly increased apoptosis in U937 cells as well as in MDA231 human breast carcinoma, A549 human lung adenocarcinoma epithelial and HCT116 human colorectal carcinoma cells. RU486 increased dose-dependent release of mitochondrial cytochrome c, and reduced the mitochondrial membrane potential (MMP, Δψm) in RU486‑treated U937 cells. We also found that overexpression of Bcl-2 completely blocked RU486-mediated apoptosis. However, reactive oxygen species signaling had no effect on RU486‑induced apoptosis. RU486 increased the phosphorylation of p38 MAPK and JNK, but p38 MAPK only was associated with RU486-mediated apoptosis. Taken together, RU486 induces apoptosis through reduction in the mitochondrial membrane potential and activation of p38 MAPK in U937 human leukemia cells.
Key words: RU486, apoptosis, caspase-3, p38 MAPK, mitochondrial membrane potential
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
JI HOON JANG et al. (2013). RU486, a glucocorticoid receptor antagonist, induces apoptosis in U937 human lymphoma cells through reduction in mitochondrial membrane potential and activation of p38 MAPK. Oncology Reports, 30(1), 506–512. doi: 10.3892/or.2013.2432
Type
Article
ISSN
1021-335X
Source
https://www.spandidos-publications.com/or/30/1/506
DOI
10.3892/or.2013.2432
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33606
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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