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Apoptosis of K562 leukemia cells by Abnobaviscum F®, a European mistletoe extract

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Author(s)
YU-KYOUNG PARKYOUNG ROK DOBYEONG-CHURL JANG
Keimyung Author(s)
Jang, Byeong ChurlDo, Young Rok
Department
Dept. of Molecular Medicine (분자의학)
Dept. of Internal Medicine (내과학)
Journal Title
Oncology Reports
Issued Date
2012
Volume
28
Issue
6
Keyword
Abnobaviscum F®caspase-9JNK-1myeloid cell leukemia-1glutathioneER stressK562
Abstract
Evidence suggests that mistletoe extract has the potential to be used as an anticancer agent. Abnobaviscum F® is a European mistletoe extract from the host tree Fraxinus. We investigated the effect of Abnobaviscum F on the growth and survival of different leukemia cell lines. Abnobaviscum F treatment strongly reduced survival and induced apoptosis of K562 (human myeloid leukemia), RPMI-8226 (human plasmacytoma) and L1210 (murine lymphocytic leukemia) cells in culture. Using K562 cells to further investigate the mechanism of action of Abnobaviscum F, we showed that Abnobaviscum F-induced cell death was associated with the activation of caspase-9, JNK-1/2 and p38 MAPK, as well as with the downregulation of Mcl-1, and inhibition of ERK-1/2 and PKB phosphorylation. Moreover, Abnobaviscum F treatment led to both a reduction of cellular glutathione (GSH) and the induction of ER stress (GRP78 and CHOP induction and eIF-2α phosphorylation). By contrast, Abnobaviscum F did not impact the expression of the DR4 and DR5 death receptors. The Abnobaviscum F-induced apoptosis of K562 cells was blocked by pretreatment with either GSH, z-VAD-fmk or SP600125. Our results, therefore, show that Abnobaviscum F induces apoptosis of K562 cells through the activation of the intrinsic caspase pathway, the phosphorylation of JNK-1, the reduction of cellular GSH, and the induction of ER stress.
Key words: Abnobaviscum F®, caspase-9, JNK-1, myeloid cell leukemia-1, glutathione, ER stress, K562
Keimyung Author(s)(Kor)
장병철
도영록
Publisher
School of Medicine
Citation
YU-KYOUNG PARK et al. (2012). Apoptosis of K562 leukemia cells by Abnobaviscum F®, a European mistletoe extract. Oncology Reports, 28(6), 2227–2232. doi: 10.3892/or.2012.2026
Type
Article
ISSN
1021-335X
Source
https://www.spandidos-publications.com/or/28/6/2227
DOI
10.3892/or.2012.2026
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33608
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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