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Pulsed Radiofrequency Application Reduced Mechanical Hypersensitivity and Microglial Expression in Neuropathic Pain Model

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Author(s)
Hea-Woon ParkSang-Ho AhnJo-Young SonSu-Jeong KimSe-Jin HwangYun-Woo ChoDong-Gyu Lee
Keimyung Author(s)
Lee, Dong Gyu
Department
Dept. of Rehabilitation Medicine (재활의학)
Journal Title
Pain Medicine
Issued Date
2012
Volume
13
Issue
9
Keyword
Pulsed RadiofrequencyNeuropathic PainMechanical HypersensitivityMicrogliaSpinal Nerve Ligation
Abstract
Objective. Pulsed radiofrequency (PRF) procedure has been used in clinical practice for the treatment of chronic neuropathic pain conditions without neuronal damage. The purpose of this study was to investigate the changes in pain response and glial expression after the application of PRF on a dorsal root ganglion (DRG) in a neuropathic pain model.

Design. A neuropathic pain model (14 female Sprague-Dawley [SD] rats; 200–250 g) was made by a unilateral L5 spinal nerve ligation (SNL) and transection on the distal side of the ligation. The development of mechanical and cold hypersensitivity on the hindpaw was established postoperative day 9 (POD 9). The rats were then randomly assigned to the PRF (+) and the PRF (−) groups. Furthermore, PRF (2 bursts/s, duration = 20 milliseconds, output voltage = 45 V) was applied on the ipsilateral DRG for 180 seconds, with a maximum temperature of 42°C, at POD 10. Pain behaviors were tested throughout the 12 days after PRF. We also examined the changes of the spinal glial expression by immunohistochemistry.

Results. Significant reduction of mechanical hypersensitivity in the PRF (+) group was observed from day 1 after a single PRF procedure and was maintained throughout the following 12 days. Immunoreactivity for OX42 in the ipsilateral dorsal horn also decreased compared with that of the PRF (−) group. However, cold hypersensitivity and glial fibrillary acidic protein (GFAP) immunoreactivity in the dorsal horn was not affected by a PRF procedure.

Conclusions. Our result demonstrated that the mechanical hypersensitivity, induced by L5 SNL, was attenuated by a PRF procedure on the ipsilateral DRG. This analgesic effect may be associated with an attenuation of the microglial activation in the dorsal horn.
Keimyung Author(s)(Kor)
이동규
Publisher
School of Medicine
Citation
Hea-Woon Park et al. (2012). Pulsed Radiofrequency Application Reduced Mechanical Hypersensitivity and Microglial Expression in Neuropathic Pain Model. Pain Medicine, 13(9), 1227–1234. doi: 10.1111/j.1526-4637.2012.01453.x
Type
Article
ISSN
1526-2375
Source
https://academic.oup.com/painmedicine/article/13/9/1227/1865372
DOI
10.1111/j.1526-4637.2012.01453.x
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33632
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학)
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