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Tigecycline inhibits proliferation of Acanthamoeba castellanii

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Author(s)
Bijay Kumar JhaIncheol SeoHyun-Hee KongSeong-Il SuhMin-Ho SuhWon-Ki Baek
Keimyung Author(s)
Suh, Seong IlSuh, Min HoBaek, Won Ki
Department
Dept. of Microbiology (미생물학)
Journal Title
Parasitology Research
Issued Date
2015
Volume
114
Issue
3
Keyword
AcanthamoebaTigecyclineATPMitochondria
Abstract
Acanthamoeba is an opportunistic protozoan parasite responsible for different diseases in humans, such as granulomatous amoebic encephalitis and amoebic keratitis. Tigecycline, a third-generation tetracycline antibiotic, has potential activity to treat most of the antibiotic resistant bacterial infections. The effects of tigecycline in eukaryotic cells as well as parasites are less well studied. In the present study, we tested the effects of tigecycline on trophozoites of Acanthamoeba castellanii. The inhibitory effect of tigecycline on Acanthamoeba was determined by resazurin reduction and trypan blue exclusion assays. We found that tigecycline significantly inhibited the growth of Acanthamoeba (46.4 % inhibition at the concentration of 100 μM) without affecting cell viability and induction of encystation, whereas other tetracycline groups of antibiotics such as tetracycline and doxycycline showed no inhibitory effects. Furthermore, tigecycline decreased cellular adenosine triphosphate (ATP) level by 26 % than the control and increased mitochondrial mass, suggesting mitochondrial dysfunction in tigecycline-treated cells. These findings suggest that mitochondrial dysfunction with decreased ATP production might play an important mechanism of tigecycline in suppression of Acanthamoeba proliferation.


Keywords
Acanthamoeba – Tigecycline – ATP – Mitochondria
Keimyung Author(s)(Kor)
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서민호
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Publisher
School of Medicine
Citation
Bijay Kumar Jha et al. (2015). Tigecycline inhibits proliferation of Acanthamoeba castellanii. Parasitology Research, 114(3), 1189–1195. doi: 10.1007/s00436-014-4302-1
Type
Article
ISSN
0932-0113
Source
https://link.springer.com/article/10.1007%2Fs00436-014-4302-1
DOI
10.1007/s00436-014-4302-1
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33637
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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