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Expression of Inhibitor-of-Apoptosis Protein (IAP) Livin by Neuroblastoma Cells: Correlation with Prognostic Factors and Outcome

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Author(s)
DAE-KWANG KIMCARLOS S. ALVARADOCARLOS R. ABRAMOWSKYLUBING GUMUXIANG ZHOUMINN-MINN SOEKEVIN SULLIVANBRADLEY GEORGEERWIN SCHEMANKEWITZHARRY W. FINDLEY
Keimyung Author(s)
Kim, Dae Kwang
Department
Dept. of Medical Genetics (의학유전학)
Journal Title
Pediatric and Developmental Pathology
Issued Date
2005
Volume
8
Issue
6
Keyword
livinprotein expressionneuroblastomaMYCNprognosis
Abstract
Livin is a recently identified member of the Inhibitor-of-Apoptosis protein (IAP) family of antiapoptosis proteins, and expression has been reported in melanoma and some types of carcinoma. We evaluated livin expression in paraffin-embedded tumor tissue from 68 patients with neuroblastoma (NB) and 7 NB cell lines by immunoperoxidase using an anti-livin monoclonal antibody. Eighteen (26.5%) of the 68 NB tumor tissues showed high livin expression, 36 (53%) showed low-intermediate expression, and 14 (20.5%) were negative. Similarly, 4 NB cell lines showed high livin expression, and 3 showed intermediate expression. In primary NB tissue, livin was observed mainly in tumor neuropil, an extension of tumor cell cytoplasm, and the cytoplasm itself. By reverse transcriptase–polymerase chain reaction, livin expression was confirmed in all 7 NB lines and in frozen tissue from 1 of 3 primary tumors examined to date, in agreement with immunohistochemical data; both livin α and β isoforms were detected. In the NB cases, we further analyzed the correlation between livin expression and clinical and biological features with established prognostic significance (i.e., age at diagnosis, stage, histology, and MYCN oncogene status), and patients’ outcome. Livin expression alone did not appear to have an effect on survival; however, patients with high livin expression and amplified MYCN had significantly decreased survival compared with patients lacking both markers or with either of these markers alone. These results suggest that (a) livin is expressed in primary and cultured neuroblastoma cells and (b) high livin expression may identify a subset of neuroblastoma patients with a particularly poor prognosis among those with MYCN amplified tumors.


Keywords
livin – protein expression – neuroblastoma – MYCN – prognosis
Keimyung Author(s)(Kor)
김대광
Publisher
School of Medicine
Citation
DAE-KWANG KIM et al. (2005). Expression of Inhibitor-of-Apoptosis Protein (IAP) Livin by Neuroblastoma Cells: Correlation with Prognostic Factors and Outcome. Pediatric and Developmental Pathology, 8(6), 621–629. doi: 10.1007/s10024-005-4108-3
Type
Article
ISSN
1093-5266
Source
https://link.springer.com/article/10.1007/s10024-005-4108-3
DOI
10.1007/s10024-005-4108-3
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33649
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Medical Genetics (의학유전학)
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