Chronic Peritoneal Inflammation by Cyanate in Rats
- Author(s)
- Kyo-Cheol Mun; Mi-Young Yeo; Sang-Pyo Kim; Hyun-Chul Kim; Chun-Sik Kwak
- Keimyung Author(s)
- Mun, Kyo Cheol; Kwak, Chun Sik; Kim, Sang Pyo; Kim, Hyun Chul
- Department
- Dept. of Biochemistry (생화학)
Dept. of Pathology (병리학)
Dept. of Internal Medicine (내과학)
Institute for Medical Science (의과학연구소)
- Journal Title
- Peritoneal Dialysis International
- Issued Date
- 2000
- Volume
- 20
- Issue
- 6
- Keyword
- Carbamylation; cyanate; peritoneum; chronic peritonitis; urea
- Abstract
- is exposed to waste products, including urea. Urea forms
cyanate spontaneously at body temperature and pH, and
cyanate carbamylates amino acids, peptides, and proteins.
Cyanate may contribute to peritoneal injury with morphological
changes in the peritoneum. To test this hypothesis,
we injected cyanate into rats.
♦ Methods: Experiments were performed in two groups
of 7 rats each. In the cyanate group, each rat received
1 mL of 1.5 μmol/L potassium cyanate dissolved in
40 mmol/L sodium bicarbonate solution intraperitoneally
each experiment day. In the control group, each rat received
1 mL of 1.5 μmol/L potassium bicarbonate instead
of potassium cyanate. The rats in both groups were anesthetized
and killed at the 85th day after the first injection.
After formalin fixation, tissue samples from abdominal
walls and livers were sliced, embedded in a standard
manner, and stained with hematoxylin and eosin.
♦ Results: Parietal peritoneum from rats in the cyanate
group showed a mild increase in the number of fibroblasts,
with collagen deposits, infiltration by mononuclear cells,
vascular congestion, round-shaped transformation of mesothelial
cells, widening of submesothelial spaces, and
abundant denudation of mesothelial cells. The visceral
peritoneum from rats in the cyanate group showed collagen
deposits with fibroblastic proliferation.
♦ Conclusions: Cyanate can induce chronic inflammation
in the peritoneum, and exposure of the peritoneum
to cyanate may contribute to peritoneal injury in patients
being treated with peritoneal dialysis.
KEY WORDS: Carbamylation; cyanate; peritoneum;
chronic peritonitis; urea.
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