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Immunomodulatory effects of aqueous-extracted Astragali radix in methotrexate-treated mouse spleen cells

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Author(s)
Young Sun LeeOk Kyung HanChan Woo ParkSeong Il SuhSang Woo ShinChae Ha YangTae Won JeonEun Sil LeeKwang Joong KimSeong Ho KimWang Keun YooHyo Jung Kim
Keimyung Author(s)
Suh, Seong Il
Department
Dept. of Microbiology (미생물학)
Journal Title
Journal of Ethnopharmacology
Issued Date
2003
Volume
84
Issue
2-3
Keyword
Astragali radixMethotrexateCytokineRT-PCRCell proliferation
Abstract
The present study was conducted to evaluate the immunomodulatory effect of aqueous-extracted Astragali radix (ARE) in methotrexate (MTX)-treated mouse spleen cells. In spleen cell proliferation assay, ARE enhanced mitogenic activity in the dose–response manner. We also investigated the effect of ARE on the reducing of immune suppression caused by MTX in mouse spleen cells. MTX decreased the spleen cell proliferation (IC50:800 μg/ml). However, ARE significantly reduced the suppression of cell proliferation by MTX in mouse spleen cells. Immunomodulatory effect of ARE were further investigated using reverse transcription polymerase chain reaction (RT-PCR). In RT-PCR, we examined the expressions of various cytokines such as IL-6, IL-1α, IL-1β, IL-12p40, GM-CSF and TNF. Enhancement of IL-1α and IL-12p40 mRNA expressions were shown in mouse spleen cells by ARE. In spite of MTX treatment, the expressions of IL-1α and IL-12p40 mRNA sustained in spleen cells. These data indicate that (1) ARE has a protective effect of immune suppression, and (2) the immunomodulatory effects of ARE may be, in part, associated with the expressions of IL-1α and IL-12p40 mRNA as well as the mitogenic effect on spleen cells.
Keimyung Author(s)(Kor)
서성일
Publisher
School of Medicine
Citation
Young Sun Lee et al. (2003). Immunomodulatory effects of aqueous-extracted Astragali radix in methotrexate-treated mouse spleen cells. Journal of Ethnopharmacology, 84(2–3), 193–198. doi: 10.1016/S0378-8741(02)00298-2
Type
Article
ISSN
0378-8741
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S0378874102002982?via%3Dihub
DOI
10.1016/S0378-8741(02)00298-2
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33778
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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