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FKBP-12 Exhibits an Inhibitory Activity on Calcium Oxalate Crystal Growth in Vitro

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Author(s)
In-Sook HanYasushi NakagawaJong-Wook ParkMin-Ho SuhSung-IL SuhSong-Woo ShinSu-Yul AhnByung-Kil Choe
Keimyung Author(s)
Park, Jong WookSuh, Min HoSuh, Seong Il
Department
Dept. of Microbiology (미생물학)
Dept. of Immunology (면역학)
Institute for Medical Science (의과학연구소)
Journal Title
Journal of Korean Medical Science
Issued Date
2002
Volume
17
Issue
1
Keyword
Tacrolimus Binding Protein 1ACalcium OxalateGrowth InhibitorNephrocalcinAntistone agent
Abstract
Urolithiasis and calcium oxalate crystal deposition diseases are still significant
medical problems. In the course of nephrocalcin cDNA cloning, we have identified
FKBP-12 as an inhibitory molecule of calcium oxalate crystal growth. gt 11
cDNA libraries were constructed from renal carcinoma tissues and screened for
nephrocalcin cDNA clones using anti-nephrocalcin antibody as a probe. Clones
expressing recombinant proteins, which appeared to be antigenically crossreactive
to nephrocalcin, were isolated and their DNA sequences and inhibitory
activities on the calcium oxalate crystal growth were determined. One of the
clone gt 11 #31-1 had a partial fragment (80 bp) of FKBP-12 cDNA as an
insert. Therefore, a full-length FKBP-12 cDNA was PCR-cloned from the gt 11
renal carcinoma cDNA library and was subcloned into an expression vector. The
resultant recombinant FKBP-12 exhibited an inhibitory activity on the calcium
oxalate crystal growth (Kd=10-7 M). Physiological effect of the extracellular FKBP-
12 was investigated in terms of macrophage activation and proinflammatory
cytokine gene induction. Extracellular FKBP-12 failed to activate macrophages
even at high concentrations. FKBP-12 seems an anti-stone molecule for the
oxalate crystal deposition disease and recurrent stone diseases.
Keimyung Author(s)(Kor)
박종욱
서민호
서성일
Publisher
School of Medicine
Citation
In-Sook Han et al. (2002). FKBP-12 Exhibits an Inhibitory Activity on Calcium Oxalate Crystal Growth in Vitro. Journal of Korean Medical Science, 17(1), 41–48. doi: 10.3346/jkms.2002.17.1.41
Type
Article
ISSN
1011-8934
DOI
10.3346/jkms.2002.17.1.41
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33793
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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