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Effect of Antisense TGF-β1 Oligodeoxynucleotides in Streptozotocin-Induced Diabetic Rat Kidney

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Author(s)
Hyo Soon JeongKwan Kyu ParkSang Pyo KimIn Jang ChoiIn Kyu LeeHyun Chul Kim
Keimyung Author(s)
Choi, In JangPark, Kwan KyuKim, Sang PyoLee, In KyuKim, Hyun Chul
Department
Dept. of Anatomy (해부학)
Dept. of Pathology (병리학)
Dept. of Internal Medicine (내과학)
Kidney Institute (신장연구소)
Journal Title
Journal of Korean Medical Science
Issued Date
2004
Volume
19
Issue
3
Keyword
Transforming Growth FactorsDiabetes MellitusOligonucleotidesStreptozotocin
Abstract
Transforming growth factor (TGF)- 1 is an important fibrogenic factor that is involved
in the pathogenesis of diabetic nephropathy. We evaluated the effect of circular
antisense TGF- 1 oligodeoxynucleotides (ODNs) on the TGF- 1 expression
in the rat mesangial cell culture and in streptozotocin (STZ)-induced diabetic rats.
Circular antisense TGF- 1 ODNs were found to be stable in rat serum, significantly
decreasing TGF- 1 mRNA expression compared with linear antisense ODNs in
the rat mesangial cell culture. Circular antisense TGF- 1 ODNs were introduced
into the tail vein of normal rats using hemagglutinating virus of Japan (HVJ)-liposomemediated
gene transfer method and were confirmed to be delivered effectively into
the kidney, liver, lungs, and spleen. To inhibit the overexpression of TGF- 1 in diabetic
kidneys, we introduced circular antisense TGF- 1 ODNs into the STZ-induced
diabetic rats. On day 13 after circular antisense TGF- 1 ODNs injection, TGF- 1
mRNA and protein expression markedly decreased and urinary TGF- 1 excretion
rate also dropped in the circular antisense TGF- 1 ODNs-treated diabetic rats.
These results suggest that circular antisense TGF- 1 ODNs may be a useful tool
for developing new therapeutic application for progressive diabetic nephropathy.
Keimyung Author(s)(Kor)
최인장
박관규
김상표
이인규
김현철
Publisher
School of Medicine
Citation
Hyo Soon Jeong et al. (2004). Effect of Antisense TGF-β1 Oligodeoxynucleotides in Streptozotocin-Induced Diabetic Rat Kidney. Journal of Korean Medical Science, 19(3), 374–383. doi: 10.3346/jkms.2004.19.3.374
Type
Article
ISSN
1011-8934
DOI
10.3346/jkms.2004.19.3.374
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33802
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
3. Research Institutues (연구소) > Kidney Institute (신장연구소)
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