계명대학교 의학도서관 Repository

Alterations of Colonic Contractility in an Interleukin-10 Knockout Mouse Model of Inflammatory Bowel Disease

Metadata Downloads
Author(s)
Kwang Bum ChoEun Su KimByung Ik JangDae Hwan KimJeong-Im SinTae Wan KimIn Hwan SongKyung Sik ParkJae Hyung ParkJoong Goo KwonSun Joo KimDae Kyu SongSeok Guen Lee
Keimyung Author(s)
Park, Jae HyungSong, Dae KyuKim, Eun SooCho, Kwang BumPark, Kyung Sik
Department
Dept. of Physiology (생리학)
Dept. of Internal Medicine (내과학)
Journal Title
Journal of Neurogastroenterology and Motility
Issued Date
2015
Volume
21
Issue
1
Keyword
ColonContractilityInflammatory bowel diseasesInterleukin-10
Abstract
Background/Aims:
Inflammatory bowel disease is commonly accompanied by colonic dysmotility and causes changes in intestinal smooth muscle
contractility. In this study, colonic smooth muscle contractility in a chronic inflammatory condition was investigated using
smooth muscle tissues prepared from interleukin-10 knockout (IL-10−/−) mice.
Methods:
Prepared smooth muscle sections were placed in an organ bath system. Cholinergic and nitrergic neuronal responses were observed
using carbachol and electrical field stimulation with L-NG-nitroarginine methyl ester (L-NAME). The expression of interstitial
cells of Cajal (ICC) networks, muscarinic receptors, neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase
(iNOS) was observed via immunofluorescent staining.
Results:
The spontaneous contractility and expression of ICC networks in the proximal and distal colon was significantly decreased in
IL-10−/− mice compared to IL-10+/+ mice. The contractility in response to carbachol was significantly decreased in the proximal
colon of IL-10−/− mice compared to IL-10+/+ mice, but no significant difference was found in the distal colon. In addition, the
expression of muscarinic receptor type 2 was reduced in the proximal colon of IL-10−/− mice. The nictric oxide-mediated relaxation
after electrical field stimulation was significantly decreased in the proximal and distal colon of IL-10−/− mice. In inflamed
colon, the expression of nNOS decreased, whereas the expression of iNOS increased.
Keimyung Author(s)(Kor)
박재형
송대규
김은수
조광범
박경식
Publisher
School of Medicine
Citation
Kwang Bum Cho et al. (2015). Alterations of Colonic Contractility in an Interleukin-10 Knockout Mouse Model of Inflammatory Bowel Disease. Journal of Neurogastroenterology and Motility, 21(1), 51–61. doi: 10.5056/jnm14008
Type
Article
ISSN
2093-0879
DOI
10.5056/jnm14008
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33831
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
Authorize & License
  • Authorize공개
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.