계명대학교 의학도서관 Repository

mRNA Expression and RNA Editing (2451 C-to-U) of IL-12 Receptor β2 in Adult Atopic Patients

Metadata Downloads
Author(s)
Eun Jin KimWon Mok LeeJung Sook HaNam Hee RyooDong Suk JeonJae Ryong Kim
Keimyung Author(s)
Lee, Won MokHa, Jung SookRyoo, Nam HeeJeon, Dong SeokKim, Jae Ryong
Department
Dept. of Laboratory Medicine (진단검사의학)
Journal Title
Journal of Korean Medical Science
Issued Date
2006
Volume
21
Issue
6
Keyword
interleukin-12 receptorIL-12R 2Gene Expression ProfilingAtopyAsthmaDermatitis, AtopicRNA Editing
Abstract
Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-γ which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially β2 subunit. In several studies, the low production of IFN-γ in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor β2 (IL-12Rβ2) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12Rβ2 mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12Rβ2 mRNA and serum total IgE or blood eosinophil count. Reduced IL-12Rβ2 mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-γ production and this may contribute to Th2-skewed immune response in atopy.
Keimyung Author(s)(Kor)
이원목
하정숙
류남희
전동석
김재룡
Publisher
School of Medicine
Citation
Eun Jin Kim et al. (2006). mRNA Expression and RNA Editing (2451 C-to-U) of IL-12 Receptor β2 in Adult Atopic Patients. Journal of Korean Medical Science, 21(6), 1070–1074. doi: 10.3346/jkms.2006.21.6.1070
Type
Article
ISSN
1011-8934
DOI
10.3346/jkms.2006.21.6.1070
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33851
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.