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Development and Immunochemical Properties of Two Monoclonal Antibodies Specific to Human Chorionic Gonadotropin

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Author(s)
You Hee KimKwan Sam Koh
Keimyung Author(s)
Kim, You Hee
Department
Dept. of Biochemistry (생화학)
Journal Title
Journal of Biochemistry & Molecular Biology
Issued Date
1999
Volume
32
Issue
5
Keyword
Human chorionic gonadotropin (hCG)Monoclonal antibody
Abstract
Using a hybridoma technique, spleen cells of Balb/c mice immunized with human chorionic gonadotropin (hCG) were fused with NS-1 mouse myeloma cells. Two hybrid cell lines, clones KS-8 and KS-19, secreting monoclonal antibodies to hCG, were isolated. KS-8 and KS-19 belong to the immunoglobulin G1 subclass. With the aid of a double-antibody radioimmunoassay, it was established that the KS-8 monoclonal antibody
recognizes an immunodeterminant of the /J-subunit of hCG, whereas the KS-19 monoclonal antibody recognizes an epitope present on the a-subunit of hCG. The KS-8 monoclonal antibody specifically reacts with human chorionic gonadotropin and shows crossreactivity of less than 0.3 % to other related human glycoprotein hormones. On the other hand, using a hemagglutination test based on antibody-induced agglutination of sheep red blood cells coated with hCG, it was shown that only the KS-19 monoclonal antibody was capable of inducing a positive reaction, although both monoclonal antibodies had similar binding capacity to the coated cells. The results from the dual screening procedures demonstrate that KS-8 and KS-19 monoclonal antibodies show high sensitivity in two different assays, and are hence useful for the qualitative and quantitative determination of hCG by both radioimmunoassay and hemagglutination inhibition tests.
Keimyung Author(s)(Kor)
김여희
Publisher
School of Medicine
Citation
You Hee Kim and Kwan Sam Koh. (1999). Development and Immunochemical Properties of Two Monoclonal Antibodies Specific to Human Chorionic Gonadotropin. Journal of Biochemistry & Molecular Biology, 32(5), 474–479.
Type
Article
ISSN
1225-8687
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33895
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
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