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Benzoyltransferase and Phenylacetyltransferase Activities in Cholestatic Rat Liver Induced by Common Bile Duct Ligation

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Author(s)
Kim, Young-JinKim, You-Hee
Keimyung Author(s)
Kim, You Hee
Department
Dept. of Biochemistry (생화학)
Journal Title
Journal of Biochemistry & Molecular Biology
Issued Date
1999
Volume
32
Issue
1
Keyword
BenzoyltransferaseCholestatic rat liverEnzyme inductionPhenylacetyltransferase
Abstract
We have investigated the effect of cholestasis on the closely related acyl-CoA:amino acid N-acyltransferase, benzoyltransferase, and phenylacetyltransferase activities in rat liver. Benzoyltransferase and phenylacetyltransferase activities in the liver cytosol, mitochondria, and microsome were investigated for a period of 42 d after common bile duct ligation. Both the mitochondrial and microsomal benzoyltransferases showed significant increase in their activities between the 1st and 7th day after common bile duct ligation, although the cytosolic benzoyltransferase activity did not show a significant change compared to the activities from the sham-operated control. The cytosolic phenylacetyltransferase activity showed a significant increase between the 1st and 2nd day, the mitochondrial activity showed a significant increase between the 2nd and 7th day, and microsomal activity showed a significant increase between the 1st and 7th day, respectively. Enzyme kinetic parameters of hepatic benzoyltransferase were analyzed using benzoyl coenzyme A as a substrate with the preparations from the 1st day post-ligation. Enzyme parameters of hepatic phenylacetyltransferase were also analyzed using phenylacetyl coenzyme A as a substrate with the preparations from the 2nd day post-ligation. The results indicated that although the values of these enzymes were about the same as the sham-operated control, the values of both enzymes increased significantly. These results, therefore, suggest that the biosynthesis of benzoyltransferase and phenylacetyltransferase has been induced in response to cholestasis.
Keimyung Author(s)(Kor)
김여희
Publisher
School of Medicine
Citation
Kim, Young-Jin and Kim, You-Hee. (1999). Benzoyltransferase and Phenylacetyltransferase Activities in Cholestatic Rat Liver Induced by Common Bile Duct Ligation. Journal of Biochemistry & Molecular Biology, 32(1), 67–71.
Type
Article
ISSN
1225-8687
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33950
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
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