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Corneal epithelial permeability after excimer laser photorefractive keratectomy

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Author(s)
Ki-San KimJong-Hwa LeeHenry F. Edelhauser
Keimyung Author(s)
Kim, Ki San
Department
Dept. of Ophthalmology (안과학)
Journal Title
Journal of Cataract & Refractive Surgery
Issued Date
1996
Volume
22
Issue
1
Abstract
Purpose: To evaluate the effect of excimer laser photorefractive keratectomy (PRK) on the corneal epithelial barrier function.

Method: Corneal uptake of 5,6 carboxyfluorescein (CF) was measured by Bernal and Ubels' method after excimer laser PRK in New Zealand white rabbits (N = 40). One cornea in each rabbit was treated, and the fellow cornea was used as a control. In both eyes, the central 7.0 mm of the corneal epithelium was removed. Myopic PRK treatments were performed at 37.75 μm (3.3 diopters [D]) and at 52.50 μm (5.0 D). The animals were euthanized and the eyes placed in CF for 5 minutes at 3 days, and at 1, 2, and 4 weeks following PRK. The corneas were then excised and dialyzed in balanced salt solution. The CF concentration in the dialysate was measured by fluorometry. Four corneas were also prepared for transmission electron microscopy using fixative containing ruthenium red.

Results: Three days after PRK, CF uptake increased in all study eyes compared with normal eyes (n = 5). One week after PRK, the control corneas showed a decreased CF uptake while the study corneas still had an increased CF uptake (P < .05). Two weeks after PRK, CF uptake in corneas with a 5.0 D ablation remained increased but decreased in corneas with a 3.3 D ablation (P < .05). Four weeks after PRK, CF uptake returned to normal in all corneas. The ruthenium red penetrated into the deeper layers of the corneal epithelium 1 week after PRK; only the superficial cell layer was stained 4 weeks after PRK.

Conclusions: Excimer laser PRK affected the corneal epithelial barrier function at 1 and 2 weeks postoperatively even though the corneal epithelium covered the ablated area. Deeper laser ablations showed higher corneal CF uptake for longer periods than shallower ablations.
Keimyung Author(s)(Kor)
김기산
Publisher
School of Medicine
Citation
Ki-San Kim et al. (1996). Corneal epithelial permeability after excimer laser photorefractive keratectomy. Journal of Cataract & Refractive Surgery, 22(1), 44–50. doi: 10.1016/S0886-3350(96)80269-0
Type
Article
ISSN
0886-3350
Source
https://www.sciencedirect.com/science/article/pii/S0886335096802690?via%3Dihub
DOI
10.1016/S0886-3350(96)80269-0
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33958
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Ophthalmology (안과학)
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