계명대학교 의학도서관 Repository

Overexpression of Par-4 Sensitizes TRAIL-Induced Apoptosis Via Inactivation of NF-kB and Akt Signaling Pathways in Renal Cancer Cells

Metadata Downloads
Author(s)
Tae-Jin LeeJi-Hoon JangHyo-Jeong NohEun-Jung ParkKyeong-Sook ChoiTaeg-Kyu Kwon
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Journal of Cellular Biochemistry
Issued Date
2010
Volume
109
Issue
5
Keyword
PAR-4TRAILDR5APOPTOSISNF-KBAKT
Abstract
The prostate-apoptosis-response-gene-4 (Par-4) is up-regulated in prostate cells undergoing programmed cell death. Furthermore, Par-4 protein has been shown to function as an effector of cell death in response to various apoptotic stimuli that trigger mitochondria and membrane receptor-mediated cell death pathways. In this study, we investigated how Par-4 modulates TRAIL-mediated apoptosis in TRAIL-resistant Caki cells. Par-4 overexpressing cells were strikingly sensitive to apoptosis induced by TRAIL compared with control cells. Par-4 overexpressing Caki cells treated with TRAIL showed an increased activation of the initiator caspase-8 and the effector caspase-3, together with an enforced cleavage of XIAP and c-FLIP. TRAIL-induced reduction of XIAP and c-FLIP protein levels in Par-4 overexpressing cells was prevented by z-VAD pretreatment. In addition, the surface DR5 protein level was increased in TRAIL-treated Par-4 overexpressing cells. Interestingly, even though a deletion of leucine zipper domain in Par-4 recovered Bcl-2 level to basal level induced by wild type Par-4, it partly decreased sensitivity to TRAIL in Caki cells. In addition, exposure of Caki/Par-4 cells to TRAIL led to reduction of phosphorylated Akt levels, but deletion of leucine zipper domain of Par-4 did not affect these phosphorylated Akt levels. In conclusion, we here provide evidence that ectopic expression of Par-4 sensitizes Caki cells to TRAIL via modulation of multiple targets, including DR5, Bcl-2, Akt, and NF-κB.
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
Tae-Jin Lee et al. (2010). Overexpression of Par-4 Sensitizes TRAIL-Induced Apoptosis Via Inactivation of NF-kB and Akt Signaling Pathways in Renal Cancer Cells. Journal of Cellular Biochemistry, 109(5), 885–895. doi: 10.1002/jcb.22504
Type
Article
ISSN
0730-2312
Source
http://lps3.onlinelibrary.wiley.com.proxy.dsmc.or.kr/doi/abs/10.1002/jcb.22504
DOI
10.1002/jcb.22504
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33971
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.