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In the Absence of Other Fc Receptors, FcγRIIIA Transmits a Phagocytic Signal That Requires the Cytoplasmic Domain of Its γ Subunit

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Author(s)
J.-G. ParkR. E. IsaacsP. ChienA. D. Schreiber
Keimyung Author(s)
Park, Jong Gu
Department
Dept. of Molecular Medicine (분자의학)
Journal Title
Journal of Clinical Investigation
Issued Date
1993
Volume
92
Issue
4
Abstract
The transmembrane isoform of Fc gamma RIII, Fc gamma RIIIA, is found on NK cells, cultured monocytes, and tissue macrophages in association with a dimer of an accessory subunit, either gamma or zeta. Functions of individual Fc receptors have been difficult to analyze due to coexpression of the receptors on hematopoietic cells and permanent cell lines expressing Fc receptors. cDNAs for the alpha and gamma subunits of Fc gamma RIIIA were cotransfected into COS-1 cells, which lack endogenous Fc receptors, to evaluate receptor-mediated phagocytosis and changes in [Ca2+]i. Transfectants both bound and phagocytosed IgG-sensitized erythrocytes and, following activation of Fc gamma RIIIA, increased [Ca2+]i. The gamma subunit was essential both for the surface expression of the receptor and for transduction of the phagocytic signal. Truncation of the gamma subunit cytoplasmic domain (amino acids 65-80) eliminated phagocytic function. Phorbol ester inhibited phagocytosis in a concentration-dependent manner, but did not affect IgG-sensitized erythrocytes binding, suggesting that a protein kinase C-dependent pathway inhibits phagocytosis. The data indicate that a tyrosine containing cytoplasmic domain within the gamma subunit is required for phagocytosis by Fc gamma RIIIA.
Keimyung Author(s)(Kor)
박종구
Publisher
School of Medicine
Citation
J.-G. Park et al. (1993). In the Absence of Other Fc Receptors, FcγRIIIA Transmits a Phagocytic Signal That Requires the Cytoplasmic Domain of Its γ Subunit. Journal of Clinical Investigation, 92(4), 1967–1973. doi: 10.1172/JCI116790
Type
Article
ISSN
0021-9738
DOI
10.1172/JCI116790
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33982
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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