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Effects of dehydroepiandrosterone on collagen and collagenase gene expression by skin fibroblasts in culture

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Author(s)
Kyu Suk LeeKwang Youl OhByung Chun Kim
Keimyung Author(s)
Lee, Kyu SukKim, Byung Chun
Department
Dept. of Dermatology (피부과학)
Journal Title
Journal of Dermatological Science
Issued Date
2000
Volume
23
Issue
2
Keyword
DHEACollagenMMPIn vitro
Abstract
Dehydroepiandrosterone (DHEA) and its sulfate (DHEA-S) are the most abundant steroids in humans whose low levels are related to aging, greater incidence of various cancers, immune dysfunction, atherosclerosis, and osteoporosis. It has been shown that collagen and collagenase gene expression decreases in fibroblasts taken from more aged donors. In this paper, to investigate the relationship between DHEA and skin aging, we examined the effects of DHEA on the regulation of collagen, collegians and stromelysin-1 genes in cultured human skin fibroblasts. In collagen assay, DHEA slightly increased collagen production in a dose-related fashion, its maximal effect occurred at 10−5 M DHEA (P>0.05). In the presence of DHEA, steady-state levels of α1 (I) procollagen mRNA increased to 1.6-fold of the non-treated group, while those of fibronectin were not. Interestingly, DHEA differently regulated collagenase and stromelysin-1 gene expression. The steady-state levels of collagenase mRNA decreased in response to DHEA by 40%, whereas those of stromelysin-1 mRNA increased up to 2.4-fold, compared to controls. Similar results were obtained for chloramphenicol acetyltransferase assay (CAT); maximal promoter activation of stromelysin-1 gene occurred at 10−6 M DHEA, 4.5-fold higher than control. CAT assay revealed that treatment with 10−5 M DHEA resulted in a strong (≈70%) inhibition of the collagenase promoter activity. In our experiments, the effects of DHEA on these gene expressions were higher at pharmacologic concentration (≥10−5 M) than those at physiologic concentration (10−8–10−6 M). This study suggests that the level of DHEA may be related to the process of skin aging through the regulation of production and degradation in extracellular matrix.
Keimyung Author(s)(Kor)
이규석
김병천
Publisher
School of Medicine
Citation
Kyu Suk Lee et al. (2000). Effects of dehydroepiandrosterone on collagen and collagenase gene expression by skin fibroblasts in culture. Journal of Dermatological Science, 23(2), 103–110. doi: 10.1016/S0923-1811(99)00094-8
Type
Article
ISSN
0923-1811
Source
http://lps3.linkinghub.elsevier.com.proxy.dsmc.or.kr/retrieve/pii/S0923-1811(99)00094-8
DOI
10.1016/S0923-1811(99)00094-8
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34013
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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