계명대학교 의학도서관 Repository

Differential down-regulation of COX-2 and MMP-13 in human skin fibroblasts by glucosamine-hydrochloride

Metadata Downloads
Author(s)
Gy-Young ParkByeong-Churl JangHua HongYu-Kyoung ParkMi-Sun ChoiNam-Hee RyuDae-Kyu SongSeong-Il SuhKi-Young Nam
Keimyung Author(s)
Choe, Mi SunRyoo, Nam HeeSong, Dae KyuSuh, Seong IlNam, Ki YoungJang, Byeong Churl
Department
Dept. of Pathology (병리학)
Dept. of Laboratory Medicine (진단검사의학)
Dept. of Physiology (생리학)
Dept. of Microbiology (미생물학)
Dept. of Dentistry (치과학)
Dept. of Molecular Medicine (분자의학)
Institute for Medical Science (의과학연구소)
Journal Title
Journal of Dermatological Science
Issued Date
2009
Volume
56
Issue
1
Keyword
Glucosamine–hydrochlorideIL-1bPMACOX-2MMP-13Human skin fibroblasts
Abstract
Background:
Evidence suggests anti-inflammatory effects of glucosamine (GS) on inflammatory diseases. COX-2 is an enzyme to produce prostaglandins. MMPs are the family of matrix metalloproteinases degradable of ECM. Excess expression of COX-2 or MMPs involves in skin inflammation.
Objective:
We evaluated whether GS–HCl modulates expression of COX-2 and/or MMPs by IL-1β or PMA in human skin fibroblasts (HSF) or keratinocytes (HaCaT).
Methods:
HSF or HaCaT cells were exposed to IL-1β or PMA without or with GS–HCl. COX-2 or MMPs protein and mRNA expression, respectively, were analyzed by Western blot and RT-PCR. MTS assay was utilized to assess the cytotoxicity of GS–HCl on HSF cells.
Results:
In HSF cells, IL-1β treatment induced COX-2 and MMP-13 expressions in association with activation of ERKs, p38 MAPK, JNKs, and NF-κB. PMA treatment also induced COX-2 and MMP-13 expressions in association with p38 MAPK activation. Of interest, treatment with GS–HCl (10 mM) led to blockage of p38 MAPK activation, accumulation of 66 kDa COX-2 protein variant (without affecting COX-2 mRNA expression), and transcriptional down-regulation of MMP-13 in the IL-1β- or PMA-treated HSF cells. Distinctly, pharmacological inhibition of p38 MAPK with SB203580 was associated with transcriptional down-regulation of COX-2 and MMP-13 in the IL-1β- or PMA-treated HSF cells. In addition, the GS–HCl-mediated COX-2 protein modification was observed in both endogenous and PMA-induced COX-2 in HaCaT cells.
Conclusions
GS–HCl differentially down-regulates COX-2 and MMP-13 expression in the IL-1β- or PMA-treated human skin fibroblasts via the p38 MAPK-independent COX-2 translational inhibition and the p38 MAPK-dependent MMP-13 transcriptional suppression, respectively.
Keimyung Author(s)(Kor)
최미선
류남희
송대규
서성일
남기영
장병철
Publisher
School of Medicine
Citation
Gy-Young Park et al. (2009). Differential down-regulation of COX-2 and MMP-13 in human skin fibroblasts by glucosamine-hydrochloride. Journal of Dermatological Science, 56(1), 43–50. doi: 10.1016/j.jdermsci.2009.06.017
Type
Article
ISSN
0923-1811
Source
http://lps3.linkinghub.elsevier.com.proxy.dsmc.or.kr/retrieve/pii/S0923-1811(09)00198-4
DOI
10.1016/j.jdermsci.2009.06.017
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34016
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dentistry (치과학)
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
Authorize & License
  • Authorize공개
  • EmbargoForever
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.