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Cadmium Induced Acute Lung Injury and TUNEL Expression of Apoptosis in Respiratory Cells

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Author(s)
Kun-Young KwonJae-Hwi JangSun-Young KwonChi-Heum ChoHoon-Kyu OhSang-Pyo Kim
Keimyung Author(s)
Kwon, Sun YoungKwon, Kun YoungOh, Hoon KyuKim, Sang PyoCho, Chi Heum
Department
Dept. of Pathology (병리학)
Dept. of Obstetrics & Gynecology (산부인과학)
Journal Title
Journal of Korean Medical Science
Issued Date
2003
Volume
18
Issue
5
Keyword
CadmiumAcute Lung InjuryTUNELApoptosis
Abstract
We examined the ultrastructural features of the lung parenchyma and the expression
of apoptosis of the respiratory cells by TUNEL technique. Male Sprague-Dawley
rats (n=30) were intra-tracheally injected with cadmium (2.5 mg/kg) into both
lungs. The light and electron microscopic features of the lung tissues were examined
on Days 1, 3, 7 and 10 after the injection of cadmium. Specimen preparations
for the light and electron microscopic TUNEL stains were performed. Ultrastructurally,
on Days 1 and 3, the alveolar spaces were filled with edematous fluid, and
desquamated type I epithelial cells. On Days 7 and 10, the alveolar spaces and
interstitium were patchy infiltrated with young fibroblasts and some collagen deposition.
The light microscopic TUNEL stain showed that apoptosis of the alveolar
cells was most prominent on Day 1, and then the number of apoptosis was markedly
decreased on Days 3, 7 and 10. The electron microscopic TUNEL stain showed
the electron dense homogenous nuclear expression, and the formation of intranuclear
blebs which protrude to the outside of nuclei. On Days 7 and 10, there
are frequent apoptotic nuclear bodies in the alveolar macrophages. We could examine
the identification of the equivocal apoptotic cells and various morphologic expression
of apoptotic nuclei on the electron microscopic TUNEL stain.
Keimyung Author(s)(Kor)
권건영
권선영
오훈규
김상표
조치흠
Publisher
School of Medicine
Citation
Kun-Young Kwon et al. (2003). Cadmium Induced Acute Lung Injury and TUNEL Expression of Apoptosis in Respiratory Cells. Journal of Korean Medical Science, 18(5), 655–662. doi: 10.3346/jkms.2003.18.5.655
Type
Article
ISSN
1011-8934
DOI
10.3346/jkms.2003.18.5.655
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34092
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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