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Role of multicolor fluorescence in situ hybridization (FISH) in simultaneous detection of probe sets for chromosome 18, X and Y in uncultured amniotic fluid cells

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Author(s)
Jong In KimJeong Ho Rhee
Keimyung Author(s)
Kim, Jong InRhee, Jeong Ho
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Institute for Cancer Research (암연구소)
Journal Title
Journal of Korean Medical Science
Issued Date
1999
Volume
14
Issue
4
Keyword
Prenatal diagnosisIn situ hybridization, fluorescenceAmniotic fluid
Abstract
Major aneuploidies diagnosed prenatally involve the autosomes 13, 18, and 21, and sex chromosomes. Fluorescence in situ hybridization (FISH) allows rapid analysis of chromosome copy number in interphase cells. The purpose of this study was to evaluate the role of multicolor fluorescence in situ hybridization in simultaneous detection of probe sets for chromosome 18, X, and Y in uncultured amniotic fluid cells as a safer alternative method for aneuploidy detection prenatally. Fifty amniotic fluid samples were analyzed by FISH and standard cytogenetics. Mean time to obtain results was three days for fluorescence in situ hybridization and 20 days for karyotype. Fluorescence in situ hybridization was informative in 43 samples (86%), and within this group, two aneuploidies were correctly identified. This evaluation demonstrates that FISH with X, Y, and 18 alpha satellite DNA probes could accurately and rapidly detect aneuploidies involving these chromosomes and could be used in any prenatal clinical laboratory.
Keimyung Author(s)(Kor)
김종인
이정호
Publisher
School of Medicine
Citation
Jong In Kim and Jeong Ho Rhee. (1999). Role of multicolor fluorescence in situ hybridization (FISH) in simultaneous detection of probe sets for chromosome 18, X and Y in uncultured amniotic fluid cells. Journal of Korean Medical Science, 14(4), 438–442. doi: 10.3346/jkms.1999.14.4.438
Type
Article
ISSN
1011-8934
DOI
10.3346/jkms.1999.14.4.438
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34123
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
3. Research Institutues (연구소) > Institute for Cancer Research (암연구소)
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